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Literature summary for 3.2.1.78 extracted from
- Application of endo-beta-1,4,D-mannanase and cellulase for the release of mannooligosaccharides from steam-pretreated spent coffee ground (2014), Appl. Biochem. Biotechnol., 172, 3538-3557.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene manA, recombinant expression in and secretion from Yarrowia lipolytica | Aspergillus aculeatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus aculeatus | the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
gene manA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme from Yarrowia lipolytica culture supernatant by ultrafiltration | Aspergillus aculeatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
recombinant enzyme in Yarrowia lipolytica is produced with an activity of 183.5 U/ml and 0.23 mg protein/ml | Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Aspergillus aculeatus | ? | - |
? | |
| additional information | the enzyme is involved in release of mannooligosaccharides from spent coffee ground cleaving the backbone at random locations in galactomannan, glucomannan, galactoglucomannan and mannan | Aspergillus aculeatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4,D-mannanase | - |
Aspergillus aculeatus |
| ManA | - |
Aspergillus aculeatus |
| ManA/HmA | - |
Aspergillus aculeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Aspergillus aculeatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 90 | activity range, profile overview | Aspergillus aculeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycerol at 30-40%, potassium sorbate/sodium benzoate at 0.2%, and sorbitol at 30-35% enhances enzyme thermostability and activity, overview | Aspergillus aculeatus |
| 60 | - |
a combination of mannanase with 0.2% sodium benzoate and 30% or 35% sorbitol presents the highest relative activity of about 150% when incubated at 60°C for 4 h, the control retains 63% of the initial activity | Aspergillus aculeatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
assay at | Aspergillus aculeatus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Aspergillus aculeatus | recombinant enzyme production is improved by 150% via supplementation with 0.2% sodium benzoate and 35% sorbitol as a preservative and stabiliser, respectively | up |
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