Any feedback?
Literature summary for 3.2.1.78 extracted from
- A new acidophilic thermostable endo-1,4-beta-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functional expression in Pichia pastoris (2014), BMC Biotechnol., 14, 90.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene poman5A, recombinant expression of C-terminally His6-tagged extracellular enzyme in and secretion from Pichia pastoris strain GS115 | Penicillium oxalicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | - |
Penicillium oxalicum |  |
| Hg2+ | - |
Penicillium oxalicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, Km values for locust bean gum, konjac mannan, and guar gum are 7.6 mg/ml, 2.1 mg/ml, and 2.3 mg/ml, respectively, pH 4.0, 80°C | Penicillium oxalicum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Penicillium oxalicum | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Penicillium oxalicum | the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose | ? | - |
? | |
| additional information | Penicillium oxalicum GZ-2 | the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | - |
gene poman5A | - |
| Penicillium oxalicum GZ-2 | - |
gene poman5A | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by nickel affinity chromatography to homogeneity | Penicillium oxalicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
84.4 U/ml of recombinant enzyme in the culture supernatant of Pichia pastoris, pH 4.0, 80°C | Penicillium oxalicum |
| 420.9 | - |
purified recombinant enzyme, locust bean gum as substrate, pH 4.0, 80°C | Penicillium oxalicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Penicillium oxalicum | ? | - |
? | |
| guar gum + H2O | - |
Penicillium oxalicum GZ-2 | ? | - |
? | |
| locust bean gum + H2O | - |
Penicillium oxalicum | ? | - |
? | |
| locust bean gum + H2O | - |
Penicillium oxalicum GZ-2 | ? | - |
? | |
| additional information | the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose | Penicillium oxalicum | ? | - |
? | |
| additional information | the enzyme catalyzes the random hydrolysis of beta-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans, main products are mannobiose, mannotriose, and mannopentaose | Penicillium oxalicum GZ-2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-mannanase | - |
Penicillium oxalicum |
| PoMan5A | - |
Penicillium oxalicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Penicillium oxalicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified recombinant His6-tagged enzyme, half-life is approximately 58 h at pH 4.0 | Penicillium oxalicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
recombinant enzyme | Penicillium oxalicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Penicillium oxalicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
