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Literature summary for 3.2.1.78 extracted from
- Cloning, over-expression and characterization of an alkali-tolerant endo-beta-1,4-mannanase from Penicillium freii F63 (2012), J. Biosci. Bioeng., 113, 710-714.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Penicillium freii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene man5F63, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning in Escherichia coli strain Trans1-T1, overexpression in Pichia pastoris strain GS115 and secretion to the culture medium | Penicillium freii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Penicillium freii | |
| Cr3+ | - |
Penicillium freii | |
| Hg2+ | - |
Penicillium freii | |
| Mg2+ | - |
Penicillium freii | |
| Ni2+ | - |
Penicillium freii | |
| Pb2+ | - |
Penicillium freii | |
| SDS | - |
Penicillium freii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km of the recombinant enzyme is 7.8 mg/ml for locust bean gum, pH 4.5, 50°C | Penicillium freii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme contains a putative 18-residue signal peptide | Penicillium freii | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Penicillium freii | |
| Cu2+ | activates | Penicillium freii | |
| Mn2+ | activates | Penicillium freii | |
| additional information | poor effects by Na+, K+, Ag+, Li+, Ca2+, Ni2+ and EDTA at 1 mM | Penicillium freii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 72000, recombinant enzyme, SDS-PAGE, x * 43000, about, sequence calculation of the mature enzyme | Penicillium freii |
| 72000 | - |
x * 72000, recombinant enzyme, SDS-PAGE, x * 43000, about, sequence calculation of the mature enzyme | Penicillium freii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium freii | I3PML9 | gene man5F63 | - |
| Penicillium freii F63 | I3PML9 | gene man5F63 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by ultrafiltration | Penicillium freii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.5 | - |
purified recombinant enzyme, pH 4.5, 50°C | Penicillium freii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| locust bean gum + H2O | - |
Penicillium freii | mannobiose + mannotriose | 52.7% mannobiose, 18.1% mannotriose and 29.2% other unidentifiable oligosaccharides | ? | |
| locust bean gum + H2O | - |
Penicillium freii F63 | mannobiose + mannotriose | 52.7% mannobiose, 18.1% mannotriose and 29.2% other unidentifiable oligosaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, recombinant enzyme, SDS-PAGE, x * 43000, about, sequence calculation of the mature enzyme | Penicillium freii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-mannanase | - |
Penicillium freii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | Penicillium freii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
purified reocmbinant enzyme, pH 4.5, 2 h, no loss of activity | Penicillium freii |
| 50 | - |
purified reocmbinant enzyme, pH 4.5, 90 min, loss of 50% activity | Penicillium freii |
| 60 | - |
purified reocmbinant enzyme, pH 4.5, 30 min, complete loss of activity | Penicillium freii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
recombinant enzyme | Penicillium freii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | more than 77% of the maximal activity in the pH range of pH 4.0 to pH 7.0 | Penicillium freii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | purified reocmbinant enzyme, 37°C, 1 h, without substrate, more than 86% of the initial activity remaning | Penicillium freii |
| 11 | 12 | purified reocmbinant enzyme, 37°C, 1 h, without substrate, more than 40% of the initial activity remaning | Penicillium freii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Penicillium freii |
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