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Literature summary for 3.2.1.78 extracted from
- Cloning, high-level expression, purification, and properties of a novel endo-beta-1,4-mannanase from Bacillus subtilis G1 in Pichia pastoris (2012), J. Microbiol. Biotechnol., 22, 331-338.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | the recombinant enzyme can be an additive in the feed for monogastric animals | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene man5A, DNA and amino acid sequence determination and analysis, recombinant expression in Pichia pastoris strain GS115 | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | - |
Bacillus subtilis |  |
| Co2+ | - |
Bacillus subtilis | |
| EDTA | - |
Bacillus subtilis | |
| ethanol | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| methanol | - |
Bacillus subtilis | |
| n-butanol | complete inhibition at 20-30% v/v | Bacillus subtilis | |
| Pb2+ | - |
Bacillus subtilis | |
| SDS | complete inhibition at 0.25-1.0% w/v | Bacillus subtilis | |
| Triton X-100 | - |
Bacillus subtilis | |
| Tween 20 | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene manA | - |
| Bacillus subtilis G1 | - |
gene manA | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris strain GS115 | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2718 | - |
purified recombinant enzyme, pH 6.5, 45°C | Bacillus subtilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
recombinant enzyme | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant enzyme, pH 5.0-9.0, 8 h, stable at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | purified recombinant enzyme, 50°C, 8 h, stable at | Bacillus subtilis |
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