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Literature summary for 3.2.1.78 extracted from
- A thermostable GH26 endo-beta-mannanase from Myceliophthora thermophila capable of enhancing lignocellulose degradation (2016), Appl. Microbiol. Biotechnol., 100, 8385-8397 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | the addition of Man26A as a supplement to the commercial enzyme mixture Celluclast 1.5 L and Novozyme 188 results in enhanced enzymatic hydrolysis of pretreated beechwood sawdust, the release of total reducing sugars and glucose is improved by 13 and 12%, respectively | Thermothelomyces thermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermothelomyces thermophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure | Thermothelomyces thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, complete loss of activity | Thermothelomyces thermophilus |  |
| additional information | presence of Ca2+, Na+, Mg2+, Mn2+, urea, and EDTA does not affect catalytic activity | Thermothelomyces thermophilus | |
| Zn2+ | 5 mM, partial loss of activity | Thermothelomyces thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carob galactomannan | Km value 2.22 mg/ml, pH 5.0, 60°C | Thermothelomyces thermophilus | |
| additional information | - |
konjac glucomannan | Km value 3.22 mg/ml, pH 5.0, 60°C | Thermothelomyces thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, 123% of initial activity | Thermothelomyces thermophilus | |
| Cu2+ | 5 mM, 121% of initial activity | Thermothelomyces thermophilus | |
| Ni2+ | 5 mM, 115% of initial activity | Thermothelomyces thermophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | G2Q4H7 | glycoside hydrolase family 26 protein | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | treatment with EndoH leads to a mass reduction of 7000 Da | Thermothelomyces thermophilus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Thermothelomyces thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 182 | - |
substrate beta-mannan, pH 5.0, 60°C | Thermothelomyces thermophilus |
| 220 | - |
substrate carob galactomannan, pH 5.0, 60°C | Thermothelomyces thermophilus |
| 312 | - |
substrate konjac glucomannan, pH 5.0, 60°C | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-mannan + H2O | - |
Thermothelomyces thermophilus | ? | - |
? | |
| carob galactomannan + H2O | - |
Thermothelomyces thermophilus | ? | - |
? | |
| konjac glucomannan + H2O | - |
Thermothelomyces thermophilus | ? | - |
? | |
| Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O | - |
Thermothelomyces thermophilus | ? | - |
? | |
| Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O | - |
Thermothelomyces thermophilus | Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose | - |
? | |
| Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O | - |
Thermothelomyces thermophilus | Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man | - |
? | |
| additional information | no activity against barley beta-glucan, birchwood xylan, and low viscosity carboxymethyl cellulose | Thermothelomyces thermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE of recombinant protein, x * 50605, calculated | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| em26a | - |
Thermothelomyces thermophilus |
| Man26A | - |
Thermothelomyces thermophilus |
| MYCTH_99077 | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life 14.4 h | Thermothelomyces thermophilus |
| 65 | - |
half-life 35.3 min | Thermothelomyces thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermothelomyces thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | more than 90% of maximum activity | Thermothelomyces thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 24 h, more than 88% residual activity | Thermothelomyces thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermothelomyces thermophilus | isoelectric focusing | - |
3.6 |
| Thermothelomyces thermophilus | calculated | - |
4.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carob galactomannan | kcat/Km value 8022 ml/mg/s, pH 5.0, 60°C | Thermothelomyces thermophilus | |
| additional information | - |
konjac glucomannan | kcat/Km value 8855 ml/mg/s, pH 5.0, 60°C | Thermothelomyces thermophilus |
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