Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Dictyoglomus turgidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 12% residual activity | Dictyoglomus turgidum | |
Cu2+ | 1 mM, 8% residual activity | Dictyoglomus turgidum | |
Fe3+ | 1 mM, 42% residual activity | Dictyoglomus turgidum | |
Mg2+ | 1 mM, 34% residual activity | Dictyoglomus turgidum | |
additional information | not inhibitory: EDTA, dithiothreitol | Dictyoglomus turgidum | |
SDS | 0.5%, 1% residual activity | Dictyoglomus turgidum | |
Triton X-100 | 0.5%, 44% residual activity | Dictyoglomus turgidum | |
Tween 20 | 0.5%, 77% residual activity | Dictyoglomus turgidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
konjac glucomannan | Km value 4.7 mg/ml, pH 5.4, 70°C | Dictyoglomus turgidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Dictyoglomus turgidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus turgidum | B8DZM3 | bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively | - |
Dictyoglomus turgidum DSM 6724 | B8DZM3 | bifunctional endo-1,4-beta-mannanase/endoglucanase, EC 3.2.1.78/EC 3.2.1.4, respectively | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
215 | - |
substrate konjac glucomannan, pH 5.4, 70°C | Dictyoglomus turgidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carob glucomannan + H2O | 13% of the activity with konjac glucomannan | Dictyoglomus turgidum | ? | - |
? | |
carob glucomannan + H2O | 13% of the activity with konjac glucomannan | Dictyoglomus turgidum DSM 6724 | ? | - |
? | |
guar galactomannan + H2O | 1.4% of the activity with konjac glucomannan | Dictyoglomus turgidum | ? | - |
? | |
guar galactomannan + H2O | 1.4% of the activity with konjac glucomannan | Dictyoglomus turgidum DSM 6724 | ? | - |
? | |
konjac glucomannan + H2O | - |
Dictyoglomus turgidum | ? | - |
? | |
konjac glucomannan + H2O | - |
Dictyoglomus turgidum DSM 6724 | ? | - |
? | |
additional information | enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans | Dictyoglomus turgidum | ? | - |
? | |
additional information | enzyme shows high endo-beta-1,4-mannanase activity versus various mannans, but low endo-beta-1,4 glucanase activity towards carboxymethylcellulose, andd negligible activity towards glucomannans | Dictyoglomus turgidum DSM 6724 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE, 1 * 40457, calculated | Dictyoglomus turgidum |
Synonyms | Comment | Organism |
---|---|---|
CelB | - |
Dictyoglomus turgidum |
Dtur_0671 | - |
Dictyoglomus turgidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Dictyoglomus turgidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
2 h, 50% residual activtiy | Dictyoglomus turgidum |
88 | - |
melting temperature | Dictyoglomus turgidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Dictyoglomus turgidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 1 h, 90% residual activity | Dictyoglomus turgidum |