Literature summary for 3.2.1.78 extracted from

Ma, L.; Ma, Q.; Cai, R.; Zong, Z.; Du, L.; Guo, G.; Zhang, Y.; Xiao, D.
Effect of beta-mannanase domain from Trichoderma reesei on its biochemical characters and synergistic hydrolysis of sugarcane bagasse (2018), J. Sci. Food Agric., 98, 2540-2547 .

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Cloned(Commentary)

Cloned (Comment)	Organism
the full-length gene (Man1) and two truncated genes (Man1DELTACBM lacking CBM domain and Man1DELTALCBM lacking linker and CBM domains) from Trichoderma reesei Rut C30 are cloned and expressed in Pichia pastoris	Trichoderma reesei

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	Q99036	-	-
Trichoderma reesei RUT C-30	Q99036	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Trichoderma reesei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
locust bean gum + H2O	-	Trichoderma reesei	?	-	?
locust bean gum + H2O	-	Trichoderma reesei RUT C-30	?	-	?

Expression

Organism	Comment	Expression
Trichoderma reesei	two truncated genes (Man1DELTACBM lacking carbohydrate-binding module domain and Man1DELTALCBM lacking linker and CBM domains) from Trichoderma reesei Rut C30 are cloned and expressed in Pichia pastoris. The expression efficiency is improved after truncating carbohydrate-binding module. Activities of Man1 and Man1DELTACBM in the culture supernatant after 168 h of induction are 34.5 and 42.9 U/ml. A value of 0.36 U/ml is detected for Man1DELTALCBM (lacking carbohydrate-binding module and linker). Man1 shows higher thermostability than Man1DELTACBM at low temperature, whereas Man1DELTACBM has a higher specificity for galactomannan (Km: 2.5 mg/ml) than Man1 (Km: 4.0 mg/mL)	additional information

General Information

General Information	Comment	Organism
metabolism	key enzyme for hydrolyzing mannan, a major constituent of hemicellulose	Trichoderma reesei

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Release 2023.1 (January 2023)