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Literature summary for 3.2.1.78 extracted from
- High-level expression and characterization of a thermophilic beta-mannanase from Aspergillus niger in Pichia pastoris (2015), Biotechnol. Lett., 37, 1853-1859 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | A2QKT4 | - |
- |
| Aspergillus niger CBS 513.88 | A2QKT4 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Aspergillus niger | mannobiose + mannotriose + mannose | - |
? |  |
| guar gum + H2O | - |
Aspergillus niger CBS 513.88 | mannobiose + mannotriose + mannose | - |
? | |
| konjaku flour + H2O | - |
Aspergillus niger | mannobiose + mannotriose + mannose | - |
? | |
| konjaku flour + H2O | - |
Aspergillus niger CBS 513.88 | mannobiose + mannotriose + mannose | - |
? | |
| locust bean gum + H2O | - |
Aspergillus niger | mannotriose + ? | - |
? | |
| locust bean gum + H2O | - |
Aspergillus niger CBS 513.88 | mannotriose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Beta-mannanase | - |
Aspergillus niger |
| Man | - |
Aspergillus niger |
| MAN-P | - |
Aspergillus niger |
| mannan endo-1,4-beta-mannosidase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 85 | the enzyme retains 60% activity at 80°C for 2 h and almost full enzyme activity remains after 4 h at 50°C. The enzyme retains 52 % enzyme activity at 85°C after 15 min | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | 11 | the enzyme is stable over the pH range 1.5-11.0. The enzyme retains 84 % of its activity at 50°C for 12 h in the pH range 3.5-9.0 | Aspergillus niger |
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