Literature summary for 3.2.1.78 extracted from

Nadaroglu, H.; Adiguzel, G.; Adiguzel, A.; Sonmez, Z.
A thermostable-endo-beta-(1,4)-mannanase from Pediococcus acidilactici (M17) purification, characterization and its application in fruit juice clarification (2017), Eur. Food Res. Technol., 243, 193-201 .

No PubMed abstract available

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Application

Application	Comment	Organism
food industry	the purified enzyme is used to clarify some fruit juices like orange, grape fruit and apple juices	Pediococcus acidilactici

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.592	-	locust bean gum	pH and temperature not specified in the publication	Pediococcus acidilactici

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Pediococcus acidilactici
Co2+	activates	Pediococcus acidilactici
Cu2+	activates	Pediococcus acidilactici
Mn2+	activates	Pediococcus acidilactici
Zn2+	activates	Pediococcus acidilactici

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	gel filtration	Pediococcus acidilactici

Organism

Organism	UniProt	Comment	Textmining
Pediococcus acidilactici	-	-	-
Pediococcus acidilactici M17	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Pediococcus acidilactici

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography and Sephacryl S200 gel filtration	Pediococcus acidilactici

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
locust bean gum + H2O	-	Pediococcus acidilactici	?	-	?
locust bean gum + H2O	-	Pediococcus acidilactici M17	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 38000, SDS-PAGE	Pediococcus acidilactici

Synonyms

Synonyms	Comment	Organism
endo-beta-(1,4)-mannanase	-	Pediococcus acidilactici

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Release 2023.1 (January 2023)