Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 123.7% activity at 1 mM | Bacillus subtilis | |
Tween 20 | 109.9% activity at 1 mM | Bacillus subtilis | |
Tween 40 | 125.4% activity at 1 mM | Bacillus subtilis | |
Tween 60 | 106.8% activity at 1 mM | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 35.7% residual activity at 5 mM | Bacillus subtilis | |
Al3+ | 84.7% residual activity at 5 mM | Bacillus subtilis | |
Cu2+ | 95.2% residual activity at 5 mM | Bacillus subtilis | |
dithiothreitol | 85.5% residual activity at 5 mM | Bacillus subtilis | |
EDTA | 26.8% residual activity at 5 mM | Bacillus subtilis | |
Fe3+ | 80.9% residual activity at 1 mM | Bacillus subtilis | |
Li+ | 96.3% residual activity at 1 mM | Bacillus subtilis | |
Mn2+ | 89.6% residual activity at 5 mM | Bacillus subtilis | |
phenylmethylsulfonyl fluoride | 80.3% residual activity at 5 mM | Bacillus subtilis | |
SDS | 79.7% residual activity at 5 mM | Bacillus subtilis | |
Zn2+ | 95% residual activity at 5 mM | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 228.3% activity at 1 mM | Bacillus subtilis | |
Co2+ | 120.7% activity at 1 mM | Bacillus subtilis | |
K+ | 119.8% activity at 1 mM | Bacillus subtilis | |
Mg2+ | 137.1% activity at 1 mM | Bacillus subtilis | |
Na+ | 121.9% activity at 1 mM | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis YH12 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Bacillus subtilis |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE column chromatography and Sephadex G75 gel filtration | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carrageenan gum + H2O | 71% activity compared to locust bean gum | Bacillus subtilis | ? | - |
? | |
carrageenan gum + H2O | 71% activity compared to locust bean gum | Bacillus subtilis YH12 | ? | - |
? | |
ceratonia siliqua gum + H2O | 46.6% activity compared to locust bean gum | Bacillus subtilis | ? | - |
? | |
fenugreek gum + H2O | 268.1% activity compared to locust bean gum | Bacillus subtilis | ? | - |
? | |
galactomannan + H2O | the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan | Bacillus subtilis | ? | - |
? | |
galactomannan + H2O | the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan | Bacillus subtilis YH12 | ? | - |
? | |
glucomannan + H2O | - |
Bacillus subtilis | ? | - |
? | |
glucomannan + H2O | - |
Bacillus subtilis YH12 | ? | - |
? | |
guar gum + H2O | 350% activity compared to locust bean gum | Bacillus subtilis | ? | - |
? | |
guar gum + H2O | 350% activity compared to locust bean gum | Bacillus subtilis YH12 | ? | - |
? | |
konjac powder + H2O | 85.1% activity compared to locust bean gum | Bacillus subtilis | mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose | - |
? | |
locust bean gum + H2O | 100% activity | Bacillus subtilis | mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose | - |
? | |
additional information | no activity on starch and xylan | Bacillus subtilis | ? | - |
? | |
additional information | no activity on starch and xylan | Bacillus subtilis YH12 | ? | - |
? | |
xanthan gum + H2O | 345.6% activity compared to locust bean gum | Bacillus subtilis | mannose + mannobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | more than 80% activity between 40 and 60°C, less than 10% activity at 65°C | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme is stable up to 60°C and retains 85% of its activity after 1.0 h of incubation | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | more than 60% activity between pH 3.0 and 8.0, no activity at pH 10.0 | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | after 1 h incubation at pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0, the enzyme retains about 68%, 82%, 93%, 95%, 90%, 68%, and 42% activity, respectively | Bacillus subtilis |