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Literature summary for 3.2.1.78 extracted from

  • Liu, H.; Gong, J.; Li, H.; Lu, Z.; Li, H.; Qian, J.; Xu, Z.; Shi, J.
    Biochemical characterization and cloning of an endo-1,4-beta-mannanase from Bacillus subtilis YH12 with unusually broad substrate profile (2015), Process Biochem., 50, 712-721 .
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 123.7% activity at 1 mM Bacillus subtilis
Tween 20 109.9% activity at 1 mM Bacillus subtilis
Tween 40 125.4% activity at 1 mM Bacillus subtilis
Tween 60 106.8% activity at 1 mM Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 35.7% residual activity at 5 mM Bacillus subtilis
Al3+ 84.7% residual activity at 5 mM Bacillus subtilis
Cu2+ 95.2% residual activity at 5 mM Bacillus subtilis
dithiothreitol 85.5% residual activity at 5 mM Bacillus subtilis
EDTA 26.8% residual activity at 5 mM Bacillus subtilis
Fe3+ 80.9% residual activity at 1 mM Bacillus subtilis
Li+ 96.3% residual activity at 1 mM Bacillus subtilis
Mn2+ 89.6% residual activity at 5 mM Bacillus subtilis
phenylmethylsulfonyl fluoride 80.3% residual activity at 5 mM Bacillus subtilis
SDS 79.7% residual activity at 5 mM Bacillus subtilis
Zn2+ 95% residual activity at 5 mM Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 228.3% activity at 1 mM Bacillus subtilis
Co2+ 120.7% activity at 1 mM Bacillus subtilis
K+ 119.8% activity at 1 mM Bacillus subtilis
Mg2+ 137.1% activity at 1 mM Bacillus subtilis
Na+ 121.9% activity at 1 mM Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis YH12
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Bacillus subtilis

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE column chromatography and Sephadex G75 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carrageenan gum + H2O 71% activity compared to locust bean gum Bacillus subtilis ?
-
?
carrageenan gum + H2O 71% activity compared to locust bean gum Bacillus subtilis YH12 ?
-
?
ceratonia siliqua gum + H2O 46.6% activity compared to locust bean gum Bacillus subtilis ?
-
?
fenugreek gum + H2O 268.1% activity compared to locust bean gum Bacillus subtilis ?
-
?
galactomannan + H2O the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan Bacillus subtilis ?
-
?
galactomannan + H2O the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan Bacillus subtilis YH12 ?
-
?
glucomannan + H2O
-
Bacillus subtilis ?
-
?
glucomannan + H2O
-
Bacillus subtilis YH12 ?
-
?
guar gum + H2O 350% activity compared to locust bean gum Bacillus subtilis ?
-
?
guar gum + H2O 350% activity compared to locust bean gum Bacillus subtilis YH12 ?
-
?
konjac powder + H2O 85.1% activity compared to locust bean gum Bacillus subtilis mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose
-
?
locust bean gum + H2O 100% activity Bacillus subtilis mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose
-
?
additional information no activity on starch and xylan Bacillus subtilis ?
-
?
additional information no activity on starch and xylan Bacillus subtilis YH12 ?
-
?
xanthan gum + H2O 345.6% activity compared to locust bean gum Bacillus subtilis mannose + mannobiose
-
?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
endo-1,4-beta-mannanase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 more than 80% activity between 40 and 60°C, less than 10% activity at 65°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme is stable up to 60°C and retains 85% of its activity after 1.0 h of incubation Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
3 8 more than 60% activity between pH 3.0 and 8.0, no activity at pH 10.0 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 after 1 h incubation at pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0, the enzyme retains about 68%, 82%, 93%, 95%, 90%, 68%, and 42% activity, respectively Bacillus subtilis