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Literature summary for 3.2.1.78 extracted from
- Kinetic properties analysis of beta-mannanase from Klebsiella oxytoca KUB-CW2-3 expressed in Escherichia coli (2018), Protein Expr. Purif., 146, 23-26 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain KMAN-3 | Klebsiella oxytoca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella oxytoca | - |
- |
- |
| Klebsiella oxytoca KUB-CW2-3 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Klebsiella oxytoca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose resin column chromatography | Klebsiella oxytoca |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 240 | - |
crude lysate, at pH 4.0 and 40°C | Klebsiella oxytoca |
| 11900 | - |
after 51fold purification, at pH 4.0 and 40°C | Klebsiella oxytoca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| defatted copra meal + H2O | - |
Klebsiella oxytoca | ? | - |
? |  |
| defatted copra meal + H2O | - |
Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
| ivory nut mannan + H2O | lowest activity | Klebsiella oxytoca | ? | - |
? | |
| ivory nut mannan + H2O | lowest activity | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
| konjac glucomannan + H2O | highest activity | Klebsiella oxytoca | ? | - |
? | |
| konjac glucomannan + H2O | highest activity | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
| locust bean gum + H2O | - |
Klebsiella oxytoca | ? | - |
? | |
| locust bean gum + H2O | - |
Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
| additional information | no activity with carboxymethyl cellulose | Klebsiella oxytoca | ? | - |
? | |
| additional information | no activity with carboxymethyl cellulose | Klebsiella oxytoca KUB-CW2-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Klebsiella oxytoca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Beta-mannanase | - |
Klebsiella oxytoca |
| endo-1,4-beta-mannanase | - |
Klebsiella oxytoca |
| KMAN-3 | recombinant enzyme | Klebsiella oxytoca |
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Release 2023.1 (January 2023)
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