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Literature summary for 3.2.1.8 extracted from

  • Damaso, M.C.T.; Almeida, M.S.; Kurtenbach, E.; Martins, O.B.; Pereira, N.Jr.; Andrade, C.M.M.C.; Albano, R.M.
    Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris (2003), Appl. Environ. Microbiol., 69, 6064-6072.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene XynA, overexpression in Pichia pastoris, method optimization, functional expression under control of the AOX1 promotor Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus O43097 gene XynA
-
Thermomyces lanuginosus IOC-4145 O43097 gene XynA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris, and native enzyme, by gel filtration Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O
-
 Thermomyces lanuginosus ?
-
 ?
xylan + H2O
-
 Thermomyces lanuginosus IOC-4145 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21900-22300, recombinant enzyme, SDS-PAGE Thermomyces lanuginosus

Synonyms

Synonyms Comment Organism
xylanase
-
 Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 native and recombinant enzyme Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 80 min, 60% remaining activity, native and recombinant enzyme Thermomyces lanuginosus
80
-
 40 min, 60% remaining activity, native and recombinant enzyme Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Thermomyces lanuginosus