Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Georis, J.; Giannotta, F.; De Buyl, E.; Granier, B.; Frere, J.M.
    Purification and properties of three endo-beta-1,4-xylanases produced by Streptomyces sp. strain S38 which differ in their ability to enhance the bleaching of kraft pulps (2000), Enzyme Microb. Technol., 26, 178-186.
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
paper production enzyme has biodelignification potential and can be used in bleaching of kraft pulps, method development, overview Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion of enzymes Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.
37500
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.
38000
-
 x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylan + H2O Streptomyces sp. delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 ?
-
 ?
xylan + H2O Streptomyces sp. S38 delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
 enzymes Xyl1, Xyl2, and Xyl3
-
Streptomyces sp. S38
-
 enzymes Xyl1, Xyl2, and Xyl3
-

Purification (Commentary)

Purification (Comment) Organism
3 enzymes to homogeneity from culture supernatant Streptomyces sp.

Source Tissue

Source Tissue Comment Organism Textmining
additional information highest enzyme production rate from culture on minimal medium at pH 7.0 and 55°C Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Streptomyces sp.
5700
-
 purified Xyl1, substrate birchwood xylan, at 50°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl arabinofuranoside + H2O Xyl2 and Xyl3 show arabinofuranosidase activity Streptomyces sp. 4-nitrophenol + arabinofuranose
-
 ?
4-nitrophenyl arabinofuranoside + H2O Xyl2 and Xyl3 show arabinofuranosidase activity Streptomyces sp. S38 4-nitrophenol + arabinofuranose
-
 ?
4-nitrophenyl cellobioside + H2O Xyl2 and Xyl3 show cellulase activity Streptomyces sp. 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl cellobioside + H2O Xyl2 and Xyl3 show cellulase activity Streptomyces sp. S38 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl xylopyranoside + H2O Xyl2 and Xyl3 show xylosidase activity Streptomyces sp. 4-nitrophenol + xylopyranose
-
 ?
additional information no activity on CM-cellulose Streptomyces sp. ?
-
 ?
additional information no activity on CM-cellulose Streptomyces sp. S38 ?
-
 ?
xylan + H2O delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 Streptomyces sp. ?
-
 ?
xylan + H2O from birchwood or oat spelt Streptomyces sp. ?
-
 ?
xylan + H2O delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 Streptomyces sp. S38 ?
-
 ?
xylan + H2O from birchwood or oat spelt Streptomyces sp. S38 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
endo-beta-1,4-xylanase
-
 Streptomyces sp.
More Xyl1 belongs to family 11, while Xyl2 and Xyl3 belong to family 10 of glycohydrolases Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 Xyl1-Xyl3 are stable for 16 h Streptomyces sp.
60
-
 half-lives: Xyl1 10 min, Xyl2 30 min, Xyl3 70 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 Xyl2 Streptomyces sp.
6 6.5 Xyl1 Streptomyces sp.
6 8 Xyl3 Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 Xyl1-Xyl3, no loss of activity in 7 days at 20°C Streptomyces sp.

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces sp. Xyl2, isoelectric focusing
-
 4.9
Streptomyces sp. Xyl3, isoelectric focusing
-
 4.9
Streptomyces sp. Xyl1, isoelectric focusing
-
 5.5