Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Cardoso, O.A.V.; Ferreira Filho, E.X.
    Purification and characterization of a novel cellulase-free xylanase from Acrophialophora nainiana (2003), FEMS Microbiol. Lett., 223, 309-314.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Acrophialophora nainiana
DTNB
-
Acrophialophora nainiana
DTT
-
Acrophialophora nainiana
L-cysteine
-
Acrophialophora nainiana
L-tryptophan
-
Acrophialophora nainiana

Inhibitors

Inhibitors Comment Organism Structure
N-bromosuccimide
-
Acrophialophora nainiana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Acrophialophora nainiana

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Acrophialophora nainiana
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500
-
2 * 27500, SDS-PAGE Acrophialophora nainiana
54000
-
gel filtration Acrophialophora nainiana

Organism

Organism UniProt Comment Textmining
Acrophialophora nainiana
-
XynIII
-
Acrophialophora nainiana XynIII
-
XynIII
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Acrophialophora nainiana

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant, 22.12fold to homogeneity by ultrafiltration and a combination of ion exchange and gel filtration chromatography Acrophialophora nainiana

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.25
-
purified enzyme Acrophialophora nainiana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O preferred substrate, soluble and insoluble substrate from oat spelt or birchwood Acrophialophora nainiana ?
-
?
xylan + H2O preferred substrate, soluble and insoluble substrate from oat spelt or birchwood Acrophialophora nainiana XynIII ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27500, SDS-PAGE Acrophialophora nainiana

Synonyms

Synonyms Comment Organism
beta-xylanase
-
Acrophialophora nainiana
xylanase
-
Acrophialophora nainiana
XynIII
-
Acrophialophora nainiana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60
-
Acrophialophora nainiana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 65 rapid decline of activity at 65°C Acrophialophora nainiana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 120 h Acrophialophora nainiana
60
-
half-life 2 h Acrophialophora nainiana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
xylan insoluble oat spelt xylan, pH 6.5, 55°C Acrophialophora nainiana
0.058
-
xylan soluble birchwood xylan, pH 6.5, 55°C Acrophialophora nainiana
0.085
-
xylan insoluble birchwood xylan, pH 6.5, 55°C Acrophialophora nainiana
0.099
-
xylan soluble oat spelt xylan, pH 6.5, 55°C Acrophialophora nainiana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Acrophialophora nainiana

pH Range

pH Minimum pH Maximum Comment Organism
4 8.5 50% of maximal activity at pH 4.0 and pH 8.5 Acrophialophora nainiana