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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of two xylanases from alkalophilic and thermophilic Bacillus licheniformis 77-2 (2006), Appl. Biochem. Biotechnol., 129-132, 289-302.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
X-I, SDS-PAGE | Bacillus licheniformis |
| 40000 | - |
X-II, SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis 77-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ethanol precipitation, Sephadex G-75 gel filtration, CM Sephadex C-50 gel filtration, Mono Q HR 5/5 column chromatography, and Q-Sepharose column chromatography | Bacillus licheniformis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
supernatant, at 60°C and pH 6.0 | Bacillus licheniformis |
| 367 | - |
X-II after 40fold purification, at 60°C and pH 6.0 | Bacillus licheniformis |
| 720 | - |
X-I after 77.4fold purification, at 60°C and pH 6.0 | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
Bacillus licheniformis | ? | X-I releases mainly xylotetraose and larger xylooligosaccharides, X-II liberates mainly xylotetraose, xylotriose, and xylobiose, whereas xylose is observed in smaller amounts | ? |  |
| birchwood xylan + H2O | - |
Bacillus licheniformis 77-2 | ? | X-I releases mainly xylotetraose and larger xylooligosaccharides, X-II liberates mainly xylotetraose, xylotriose, and xylobiose, whereas xylose is observed in smaller amounts | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| X-I | - |
Bacillus licheniformis |
| X-II | - |
Bacillus licheniformis |
| xylanase | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
X-I | Bacillus licheniformis |
| 75 | - |
X-II | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
X-I and X-II are 100% stable after 1 h at 40°C | Bacillus licheniformis |
| 50 | - |
X-I retains more than 90% of hydrolytic activity after 1 h of heating at 50°C | Bacillus licheniformis |
| 60 | - |
X-II retains more than 90% of hydrolytic activity after 1 h of heating at 60°C | Bacillus licheniformis |
| 90 | - |
X-I presents 50% activity at 90°C, and X-II presents 80% activity at the same temperature | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
X-I | Bacillus licheniformis |
| 8 | 10 | X-II | Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
X-I and X-II retain more than 75% of hydrolytic activity up to pH 11.0 | Bacillus licheniformis |
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