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Literature summary for 3.2.1.8 extracted from

  • Bourgois, T.M.; Nguyen, D.V.; Sansen, S.; Rombouts, S.; Belien, T.; Fierens, K.; Raedschelders, G.; Rabijns, A.; Courtin, C.M.; Delcour, J.A.; Van Campenhout, S.; Volckaert, G.
    Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types (2007), J. Biotechnol., 130, 95-105.
    View publication on PubMed

Application

Application Comment Organism
food industry improvement of cereal-based industrial processing by endoxylanase enzymes insensitive towards inhibitors Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli WK6 cells Aspergillus niger
expresssed in Escherichia coli WK6 cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D11F/R122D no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
G12 no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
G12K decreased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger
G12W no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
N35D increased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger
Q127E specific activity is lower than 5% compared to the wild type enzyme Aspergillus niger
Q127K no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
Q127L specific activity is lower than 5% compared to the wild type enzyme Aspergillus niger
R112H wild type comparable sensitivity to TAXI-I Aspergillus niger
R112Y no inhibition by Triticum aestivum xylanase inhibitor-I Aspergillus niger
W9Y mutant enzyme is insensitive for Triticum aestivum xylanase inhibitor-II Aspergillus niger
W9Y/N35D increased sensitivity to Triticum aestivum xylanase inhibitor-I and insensitive for Triticum aestivum xylanase inhibitor-II Aspergillus niger
Y174W increased sensitivity to Triticum aestivum xylanase inhibitor-I Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Triticum aestivum endoxylanase inhibitor-I
-
Bacillus subtilis
Triticum aestivum endoxylanase inhibitor-II
-
Bacillus subtilis
Triticum aestivum xylanase inhibitor-I
-
Aspergillus niger
Triticum aestivum xylanase inhibitor-II
-
Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Bacillus subtilis P18429
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni-NTA column Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
91
-
mutant Q127E Bacillus subtilis
245
-
mutant Q127L Bacillus subtilis
594
-
mutant R112Y Bacillus subtilis
619
-
mutant Q127K Bacillus subtilis
1655
-
mutant D11F/R122D Bacillus subtilis
3707
-
mutant W9Y Bacillus subtilis
3722
-
mutant R112H Bacillus subtilis
4031
-
mutant N35D Bacillus subtilis
4890
-
mutant G12R Bacillus subtilis
5072
-
mutant G12W Bacillus subtilis
5152
-
mutant W9Y/N35D Bacillus subtilis
5234
-
mutant G12K Bacillus subtilis
5779
-
mutant Y174W Bacillus subtilis
5890
-
wild-type Bacillus subtilis
5890
-
wild type enzyme, at pH 7.0 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O
-
Aspergillus niger ?
-
?
arabinoxylan + H2O
-
Bacillus subtilis ?
-
?
birchwood xylan + H2O
-
Aspergillus niger ?
-
?
birchwood xylan + H2O
-
Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
BSXY
-
Aspergillus niger
BSXY
-
Bacillus subtilis
endoxylanase
-
Aspergillus niger
family 11 endoxylanase
-
Bacillus subtilis
XynA
-
Aspergillus niger
XynA
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.039
-
birchwood xylan wild-type Bacillus subtilis
0.039
-
birchwood xylan wild type enzyme, at pH 7.0 Aspergillus niger
2
-
birchwood xylan mutant W9Y/N35D Bacillus subtilis
8.8
-
birchwood xylan mutant R112Y Bacillus subtilis
14.6
-
birchwood xylan mutant Q127K Bacillus subtilis
21
-
birchwood xylan mutant Y174W Bacillus subtilis
39
-
birchwood xylan mutant D11F/R122D Bacillus subtilis
50.7
-
birchwood xylan mutant N35D Bacillus subtilis
65.4
-
birchwood xylan mutant G12W Bacillus subtilis
71.6
-
birchwood xylan mutant W9Y/N35D Bacillus subtilis
88.7
-
birchwood xylan mutant W9Y Bacillus subtilis
111.2
-
birchwood xylan mutant Y174W Bacillus subtilis
140.1
-
birchwood xylan wild-type Bacillus subtilis
140.1
-
birchwood xylan wild type enzyme, at pH 7.0 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aspergillus niger
7
-
for most mutants the pH optimum is around 5.5-6.0 Bacillus subtilis