Application | Comment | Organism |
---|---|---|
food industry | improvement of cereal-based industrial processing by endoxylanase enzymes insensitive towards inhibitors | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli WK6 cells | Aspergillus niger |
expresssed in Escherichia coli WK6 cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D11F/R122D | no inhibition by Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
G12 | no inhibition by Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
G12K | decreased sensitivity to Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
G12W | no inhibition by Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
N35D | increased sensitivity to Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
Q127E | specific activity is lower than 5% compared to the wild type enzyme | Aspergillus niger |
Q127K | no inhibition by Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
Q127L | specific activity is lower than 5% compared to the wild type enzyme | Aspergillus niger |
R112H | wild type comparable sensitivity to TAXI-I | Aspergillus niger |
R112Y | no inhibition by Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
W9Y | mutant enzyme is insensitive for Triticum aestivum xylanase inhibitor-II | Aspergillus niger |
W9Y/N35D | increased sensitivity to Triticum aestivum xylanase inhibitor-I and insensitive for Triticum aestivum xylanase inhibitor-II | Aspergillus niger |
Y174W | increased sensitivity to Triticum aestivum xylanase inhibitor-I | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triticum aestivum endoxylanase inhibitor-I | - |
Bacillus subtilis | |
Triticum aestivum endoxylanase inhibitor-II | - |
Bacillus subtilis | |
Triticum aestivum xylanase inhibitor-I | - |
Aspergillus niger | |
Triticum aestivum xylanase inhibitor-II | - |
Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Bacillus subtilis | P18429 | - |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA column | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
91 | - |
mutant Q127E | Bacillus subtilis |
245 | - |
mutant Q127L | Bacillus subtilis |
594 | - |
mutant R112Y | Bacillus subtilis |
619 | - |
mutant Q127K | Bacillus subtilis |
1655 | - |
mutant D11F/R122D | Bacillus subtilis |
3707 | - |
mutant W9Y | Bacillus subtilis |
3722 | - |
mutant R112H | Bacillus subtilis |
4031 | - |
mutant N35D | Bacillus subtilis |
4890 | - |
mutant G12R | Bacillus subtilis |
5072 | - |
mutant G12W | Bacillus subtilis |
5152 | - |
mutant W9Y/N35D | Bacillus subtilis |
5234 | - |
mutant G12K | Bacillus subtilis |
5779 | - |
mutant Y174W | Bacillus subtilis |
5890 | - |
wild-type | Bacillus subtilis |
5890 | - |
wild type enzyme, at pH 7.0 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylan + H2O | - |
Aspergillus niger | ? | - |
? | |
arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? | |
birchwood xylan + H2O | - |
Aspergillus niger | ? | - |
? | |
birchwood xylan + H2O | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BSXY | - |
Aspergillus niger |
BSXY | - |
Bacillus subtilis |
endoxylanase | - |
Aspergillus niger |
family 11 endoxylanase | - |
Bacillus subtilis |
XynA | - |
Aspergillus niger |
XynA | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.039 | - |
birchwood xylan | wild-type | Bacillus subtilis | |
0.039 | - |
birchwood xylan | wild type enzyme, at pH 7.0 | Aspergillus niger | |
2 | - |
birchwood xylan | mutant W9Y/N35D | Bacillus subtilis | |
8.8 | - |
birchwood xylan | mutant R112Y | Bacillus subtilis | |
14.6 | - |
birchwood xylan | mutant Q127K | Bacillus subtilis | |
21 | - |
birchwood xylan | mutant Y174W | Bacillus subtilis | |
39 | - |
birchwood xylan | mutant D11F/R122D | Bacillus subtilis | |
50.7 | - |
birchwood xylan | mutant N35D | Bacillus subtilis | |
65.4 | - |
birchwood xylan | mutant G12W | Bacillus subtilis | |
71.6 | - |
birchwood xylan | mutant W9Y/N35D | Bacillus subtilis | |
88.7 | - |
birchwood xylan | mutant W9Y | Bacillus subtilis | |
111.2 | - |
birchwood xylan | mutant Y174W | Bacillus subtilis | |
140.1 | - |
birchwood xylan | wild-type | Bacillus subtilis | |
140.1 | - |
birchwood xylan | wild type enzyme, at pH 7.0 | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aspergillus niger |
7 | - |
for most mutants the pH optimum is around 5.5-6.0 | Bacillus subtilis |