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Literature summary for 3.2.1.8 extracted from
- Understanding the structural basis for substrate and inhibitor recognition in eukaryotic GH11 xylanases (2008), J. Mol. Biol., 375, 1293-1305.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Neocallimastix patriciarum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| enzyme bound to ferulic acid-1,5-arabinofuranose-alpha-1,3-xylotriose, hanging drop vapour diffusion method, with 0.1 M cadmium chloride, 0.1 M sodium acetate (pH 4.6) and 30% (w/v) polyethylene glycol 400 | Neocallimastix patriciarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by XIP-I | Neocallimastix patriciarum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
4-nitrophenyl-beta-D-xylotrioside | in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5) | Neocallimastix patriciarum |  |
| 15 | - |
4-nitrophenyl-beta-D-xylobioside | in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5) | Neocallimastix patriciarum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neocallimastix patriciarum | P29127 | bifunctional endo-1,4-beta-xylanase A precursor | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q10 anion exchange column chromatography | Neocallimastix patriciarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-xylobioside + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| 4-nitrophenyl beta-D-xylotrioside + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| oat spelt xylan + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| xylohexaose + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| xylopentaose + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| xylotetraose + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
| xylotriose + H2O | - |
Neocallimastix patriciarum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GH11 xylanase | - |
Neocallimastix patriciarum |
| xylanase | - |
Neocallimastix patriciarum |
| Xyn11A | - |
Neocallimastix patriciarum |
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