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Literature summary for 3.2.1.8 extracted from

  • Chiku, K.; Uzawa, J.; Seki, H.; Amachi, S.; Fujii, T.; Shinoyama, H.
    Characterization of a novel polyphenol-specific oligoxyloside transfer reaction by a family 11 xylanase from Bacillus sp. KT12 (2008), Biosci. Biotechnol. Biochem., 72, 2285-2293.
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
additional information the Bacillus sp. KT12 xylanolytic enzyme is a suitable enzyme for the synthesis of polyphenyl beta-oligoxylosides Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ inhibits hydrolysis activity Bacillus subtilis
Ca2+ completely inhibits hydrolysis activity Bacillus subtilis
Co2+ inhibits hydrolysis activity Bacillus subtilis
Cu2+ inhibits hydrolysis activity Bacillus subtilis
EDTA completely inhibits hydrolysis activity Bacillus subtilis
Mg2+ inhibits hydrolysis activity Bacillus subtilis
Mn2+ inhibits hydrolysis activity Bacillus subtilis
additional information hydrolytic activity almost entirely inhibits at acidic pH Bacillus subtilis
Zn2+ completely inhibits hydrolysis activity Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ strongly activates transxylosylation activity Bacillus subtilis
additional information other metalions than Fe2+ have an insignificant effect on transxylosylation activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 1 * 20000, SDS-PAGE and HPLC Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-
Aspergillus niger IFO 31628
-
-
-
Bacillus subtilis Q45VU2
-
-
Bacillus subtilis KT12 Q45VU2
-
-
Penicillium expansum
-
-
-
Penicillium expansum IFO 8800
-
-
-
Trichoderma viride
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate fractionation, centrifugation and gel filtration, 7.7fold Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methoxyphenol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
ascorbic acid + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
beech wood xylan + H2O
-
 Aspergillus niger ?
-
 ?
beech wood xylan + H2O
-
 Trichoderma viride ?
-
 ?
beech wood xylan + H2O
-
 Penicillium expansum ?
-
 ?
beech wood xylan + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
beech wood xylan + H2O
-
 Penicillium expansum IFO 8800 ?
-
 ?
beech wood xylan + H2O
-
 Aspergillus niger IFO 31628 ?
-
 ?
caffeic acid + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
catechin + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
catechol + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
DOPA + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
dopamine + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
fructose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
gallic acid + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
glucose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
glucose + H2O weak transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
hydroquinone + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
lactose + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
lactose + H2O strong transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
maltose + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
maltose + H2O strong transxylosylation activity Bacillus subtilis KT12 ?
-
 ?
mannitol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
additional information displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) Bacillus subtilis ?
-
 ?
additional information displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol) Bacillus subtilis KT12 ?
-
 ?
nutrient broth + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
phloroglucinol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
pyrogallol + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
resorcinol + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
starch + H2O strong transxylosylation activity Bacillus subtilis ?
-
 ?
sucrose + H2O strong transxylosylation activity Bacillus subtilis D-fructose + D-glucose
-
 ?
sucrose + H2O strong transxylosylation activity Bacillus subtilis KT12 D-fructose + D-glucose
-
 ?
xylooligosaccharide + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?
xylose + H2O weak transxylosylation activity Bacillus subtilis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 20000, SDS-PAGE and HPLC Bacillus subtilis

Synonyms

Synonyms Comment Organism
endoxylanase
-
 Aspergillus niger
endoxylanase
-
 Trichoderma viride
endoxylanase
-
 Penicillium expansum
endoxylanase
-
 Bacillus subtilis
xylanase
-
 Aspergillus niger
xylanase
-
 Trichoderma viride
xylanase
-
 Penicillium expansum
xylanase
-
 Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 transxylosylation and hydrolysis activities are optimal at temperatures of 60°C and 40°C, respectively Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 near constant transxylosylation activity Bacillus subtilis

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus subtilis IEF-PAGE
-
 7.5