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Literature summary for 3.2.1.8 extracted from

  • Watanabe, S.; Viet, D.N.; Kaneko, J.; Kamio, Y.; Yoshida, S.
    Cloning, expression, and transglycosylation reaction of Paenibacillus sp. strain W-61 xylanase 1 (2008), Biosci. Biotechnol. Biochem., 72, 951-958.
    View publication on PubMed

Application

Application Comment Organism
industry oligoglycosides have excellent properties as both surfactants and biodegradability enhancers, whereby Xyn1 is probably useful for such synthesis, since Xyn1 has high transglycosylation activity Paenibacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
into vector pUC119 and transformation of Escherichia coli DH5alpha. Into the NcoI site of pTrc99A and expressed in Escherichia coli BL21 CodonPlusR-RIL Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20280
-
sequence analysis Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration to homogeneity with a purification yield of 11.3% Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
130.4
-
purified enzyme toward oat spelt xylan Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with xylobiose and xylotriose Paenibacillus sp. ?
-
?
oat spelt xylan + H2O
-
Paenibacillus sp. ?
-
?
xyloheptaose + H2O
-
Paenibacillus sp. xylotriose + ?
-
?
xylohexaose + H2O
-
Paenibacillus sp. 2 xylotriose
-
?
xylopentaose + H2O
-
Paenibacillus sp. xylobiose + xylotriose + xylotetraose
-
?
xylotetraose + H2O
-
Paenibacillus sp. xylobiose + xylotriose
-
?

Synonyms

Synonyms Comment Organism
xylanase 1
-
Paenibacillus sp.
Xyn1
-
Paenibacillus sp.