Literature summary for 3.2.1.8 extracted from

Stephens, D.E.; Singh, S.; Permaul, K.
Error-prone PCR of a fungal xylanase for improvement of its alkaline and thermal stability (2009), FEMS Microbiol. Lett., 293, 42-47.

Search for more literature for 3.2.1.8

Application

Application	Comment	Organism
industry	variants with improved thermal and alkaline stability are ideal candidates for DNA shuffling experiments to produce a robust xylanase for industrial application	Thermomyces lanuginosus

Cloned(Commentary)

Cloned (Comment)	Organism
xynA cloned from a genomic library into pBluescript. Extraneous fungal DNA upstream and downstream of xynA and the lacZ fusion partner removed by PCR. After PCR, cleavage and recloning, and the plasmid transformed into Escherichia coli XL1 Blue MRF'	Thermomyces lanuginosus

Protein Variants

Protein Variants	Comment	Organism
A54T	when exposed to 80°C for 90 min the mutant displays a low stability and retains only 10% of its activity. It is extremely alkali tolerant. After 90 min at pH 10 it retains 93% of its activity. It has catalytic activity almost comparable to the wild-type	Thermomyces lanuginosus
K30E/W40R/T57A/K80R	thermostable mutant, when exposed to 80°C for 90 min it displays 75% retention of its total activity. The mutant loses nearly 60% of its activity under extremely alkaline conditions (after 90 min at pH 10). It has a much lower activity as compared to the wild-type	Thermomyces lanuginosus

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	native XynA loses 80% activity after 90 min at 80°C and 70% activity at pH 10	Thermomyces lanuginosus

Organism

Organism	UniProt	Comment	Textmining
Thermomyces lanuginosus	-	strain DSM 5826	-

Synonyms

Synonyms	Comment	Organism
xylanase	-	Thermomyces lanuginosus
XynA	-	Thermomyces lanuginosus

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Release 2023.1 (January 2023)