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Literature summary for 3.2.1.8 extracted from

  • Zhou, J.; Huang, H.; Meng, K.; Shi, P.; Wang, Y.; Luo, H.; Yang, P.; Bai, Y.; Yao, B.
    Cloning of a new xylanase gene from Streptomyces sp. TN119 using a modified thermal asymmetric interlaced-PCR specific for GC-rich genes and biochemical characterization (2010), Appl. Biochem. Biotechnol., 160, 1277-1292.
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activates 1.6fold at 10 mM Streptomyces sp. TN119

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA119, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3), the recombinant xylanase is secreted to the culture supernatant Streptomyces sp. TN119

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 19% inhibition at 10mM Streptomyces sp. TN119
Cu2+ 33% inhibition at 10mM Streptomyces sp. TN119
Hg2+ complete inhibition at 1 mM Streptomyces sp. TN119
Mn2+ 19% inhibition at 5 mM, 60% at 10mM Streptomyces sp. TN119
additional information no or poor inhibition by Ni2+, Cr3+, Mg2+, Li+, Fe3+, K+, Na+, Ca2+, and EDTA Streptomyces sp. TN119
Pb2+ 18% inhibition at 10mM Streptomyces sp. TN119
SDS 84% inhibition at 5 mM, complete at 10mM Streptomyces sp. TN119

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no or poor inhibition by Ni2+, Cr3+, Mg2+, Li+, Fe3+, K+, Na+, Ca2+, and EDTA Streptomyces sp. TN119
Zn2+ activates 1.2fold at 10 mM Streptomyces sp. TN119

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 x * 38000, recombinant enzyme, SDS-PAGE Streptomyces sp. TN119

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oat-spelt xylan + H2O Streptomyces sp. TN119
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. TN119 C6FX34 isolated from the gut of Batocera horsfieldi larvae collected from oak trees, gene xynA119
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) culture supernatant to homogeneity by nickel affinity chromatography Streptomyces sp. TN119

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
 recombinant enzyme, culture supernatant Streptomyces sp. TN119
57.9
-
 purified recombinant enzyme, substrate oat-spelt xylan Streptomyces sp. TN119

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oat-spelt xylan + H2O
-
 Streptomyces sp. TN119 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 38000, recombinant enzyme, SDS-PAGE Streptomyces sp. TN119

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the glycosyl hydrolases family 10, GH 10 Streptomyces sp. TN119
XynA119
-
 Streptomyces sp. TN119

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 recombinant enzyme Streptomyces sp. TN119

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant enzyme, 1 h, 95% remaining activity, rapid decrease in activity above 60°C Streptomyces sp. TN119
65
-
 purified recombinant enzyme, 1 h, 70% remaining activity Streptomyces sp. TN119
70
-
 purified recombinant enzyme, 1 h, 45% remaining activity Streptomyces sp. TN119

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
49.2
-
 oat-spelt xylan pH 6.5, 60°C Streptomyces sp. TN119

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 recombinant enzyme Streptomyces sp. TN119

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 over 60% of maximal activity within this range, recombinant enzyme Streptomyces sp. TN119

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 purified recombinant enzyme, 1 h, 37°C, 85% remaining activity Streptomyces sp. TN119