Application | Comment | Organism |
---|---|---|
industry | XYN10G5 a good candidate for application in the animal feed industry | Phialophora sp. CGMCC 3328 |
Cloned (Comment) | Organism |
---|---|
gene xyn10G5, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Phialophora sp. CGMCC 3328 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 | |
Co2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
Cu2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
Hg2+ | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 | |
additional information | after treatment with pepsin and trypsin at 37°C for 1 h, XYN10G retains 64.7% and 100% activity, respectively | Phialophora sp. CGMCC 3328 | |
Ni2+ | strong inhibition of XYN10G at 1 mM | Phialophora sp. CGMCC 3328 | |
SDS | complete inhibition of XYN10G at 5 mM | Phialophora sp. CGMCC 3328 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+, K+, Li+, Ca2+, Mg2+, Mn2+ and EDTA, have little or no effect on the enzyme activity | Phialophora sp. CGMCC 3328 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phialophora sp. CGMCC 3328 | F2VRY7 | gene xyn10G5 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 by ultrafiltration, anion exchange chromatography, and gel filtration | Phialophora sp. CGMCC 3328 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
207.2 | - |
purified recombinant XYN10G5, substrate oat spelt xylan, pH 4.0, 70°C | Phialophora sp. CGMCC 3328 |
350.6 | - |
purified recombinant XYN10G5, substrate soluble wheat arabinoxylan, pH 4.0, 70°C | Phialophora sp. CGMCC 3328 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birch wood xylan + H2O | - |
Phialophora sp. CGMCC 3328 | ? | - |
? | |
additional information | composition of the hydrolysis products from oat spelt xylan, birchwood xylan, and soluble wheat arabinoxylan are 43.3% xylose and 56.7% xylobiose, 41.0% xylose and 59.0% xylobiose, and 42.4% xylose, and 57.6% xylobiose, respectively | Phialophora sp. CGMCC 3328 | ? | - |
? | |
oat spelt xylan + H2O | - |
Phialophora sp. CGMCC 3328 | ? | - |
? | |
soluble wheat arabinoxylan + H2O | best substrate of XYN10G5, Under simulated gastric conditions, XYN10G5 is stable and releases more reducing sugars from soluble wheat arabinoxylan | Phialophora sp. CGMCC 3328 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, recombinant enzyme, SDS-PAGE, x * 40000, about, sequence calculation | Phialophora sp. CGMCC 3328 |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xylanase | - |
Phialophora sp. CGMCC 3328 |
More | the enzyme belongs to the glycoside hydrolase domain family 10 | Phialophora sp. CGMCC 3328 |
XYN10G5 | - |
Phialophora sp. CGMCC 3328 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Phialophora sp. CGMCC 3328 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
XYN10G, about 90% of activity remaining after 1 h, 37°C | Phialophora sp. CGMCC 3328 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Phialophora sp. CGMCC 3328 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | XYN10G, 70% of maximal activity within this range | Phialophora sp. CGMCC 3328 |