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Literature summary for 3.2.1.8 extracted from
- Characterization of a bifunctional xylanase/endoglucanase from yak rumen microorganisms (2011), Appl. Microbiol. Biotechnol., 90, 1933-1942.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | uncultured microorganism |  |
| Co2+ | activates | uncultured microorganism | |
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | RuCelA can produce xylo-oligosaccharides and cell-oligosaccharides in the continuous saccharification of pretreated rice straw, which can be further degraded into fermentable sugars. Therefor, the bifunctional RuCelA distinguishes itself as an ideal candidate for industrial application | uncultured microorganism |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21/pET21a | uncultured microorganism |
| RuCelA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | uncultured microorganism |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM, activity decreased to 45% | uncultured microorganism | |
| Co2+ | 10 mM, activity decreased to 60% | uncultured microorganism | |
| Cu2+ | 10 mM, activity decreased to 20% | uncultured microorganism | |
| Mg2+ | 10 mM, activity decreased to 70% | uncultured microorganism | |
| Zn2+ | 10 mM, activity decreased to 15% | uncultured microorganism | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | uncultured microorganism | |
| additional information | - |
xylan | 0.58 g/l at 65°C, pH 7.0 | uncultured microorganism | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60600 | - |
calculated mass | uncultured microorganism |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | uncultured microorganism | RuCelA is a bifunctional xylanase/endoglucanase showing activity against xylan and carboxymethyl cellulose. Using xylan and barley glucan as substrates, RuCelA displays obvious synergistic effects with beta-1,4-xylosidase and beta-1,4-glucosidase. RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | E2DQY5 | from Bos mutus rumen | - |
| uncultured microorganism | E2DQY5 | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the cloned protein from the recombinant strain BL21/pET21a-RuCelA is purified using Ni-NTA resin | uncultured microorganism |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens | uncultured microorganism | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 264.1 | - |
purified recombinant enzyme, pH 7.0, 65°C | uncultured microorganism |
| 264.1 | - |
xylan as substrate, pH 7.0, 65°C | uncultured microorganism |
| 805 | - |
lichenin as substrate, pH 7.0, 65°C | uncultured microorganism |
| 938 | - |
barley glucan as substrate, pH 7.0, 65°C | uncultured microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley glucan + H2O | - |
uncultured microorganism | ? | the main end-products are monosaccharides, disaccharides, trisacchraides, and tetrasaccharides | ? | |
| birch wood xylan + H2O | - |
uncultured microorganism | ? | - |
? | |
| lichenin + H2O | - |
uncultured microorganism | ? | - |
? | |
| additional information | RuCelA is a bifunctional xylanase/endoglucanase showing activity against xylan and carboxymethyl cellulose. Using xylan and barley glucan as substrates, RuCelA displays obvious synergistic effects with beta-1,4-xylosidase and beta-1,4-glucosidase. RuCelA can produce xylo-oligosaccharides and cello-oligosaccharides in the continuous saccharification of pretreated rice straw | uncultured microorganism | ? | - |
? | |
| additional information | no activitiy with beta-1,3-glucan, laminarin, or Avicel as substrates | uncultured microorganism | ? | - |
? | |
| xylan + H2O | - |
uncultured microorganism | ? | the main end-products are monosaccharides, disaccharides, trisacchraides, and tetrasaccharides, mainly xylose, xylobiose, and xylotetrose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60600, about, sequence calculation | uncultured microorganism |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bifunctional xylanase/endoglucanase | RuCelA, a bifunctional xylanase/endoglucanase, EC 3.2.1.8 and EC 3.2.1.73 | uncultured microorganism |
| RuCelA | - |
uncultured microorganism |
| xylanase | - |
uncultured microorganism |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
uncultured microorganism |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | activity range | uncultured microorganism |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
xylan as substrate, remains stable after treatment for 1 h | uncultured microorganism |
| 30 | 50 | purified recombinant RuCelA, 1 h, stable | uncultured microorganism |
| 40 | - |
xylan as substrate, remains stable after treatment for 1 h | uncultured microorganism |
| 50 | - |
xylan as substrate, remains stable after treatment for 1 h | uncultured microorganism |
| 60 | - |
purified recombinant RuCelA, 1 h, 60% activity remaining | uncultured microorganism |
| 60 | - |
xylan as substrate, remains 60% activity after treatment for 1 h | uncultured microorganism |
| 70 | - |
purified recombinant RuCelA, 1 h, almost no activity remaining | uncultured microorganism |
| 70 | - |
xylan as substrate, almost loses its activity after treatment for 1 h | uncultured microorganism |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
uncultured microorganism |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | activity range | uncultured microorganism |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified recombinant RuCelA, 24 h, 55% activity remaining | uncultured microorganism |
| 3 | - |
xylan as substrate, loses 45% of its activity after incubation for 24 h | uncultured microorganism |
| 4 | 9 | purified recombinant RuCelA, 24 h, 80% activity remaining | uncultured microorganism |
| 4 | 9 | xylan as substrate, remains more than 80% of its activity | uncultured microorganism |
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