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Literature summary for 3.2.1.8 extracted from

  • Anasontzis, G.E.; Zerva, A.; Stathopoulou, P.M.; Haralampidis, K.; Diallinas, G.; Karagouni, A.D.; Hatzinikolaou, D.G.
    Homologous overexpression of xylanase in Fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (CBP) of lignocellulosics (2011), J. Biotechnol., 152, 16-23.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the endo-beta-1,4-xylanase 2 gene is cloned from strain F3, then incorporated into the Fusarium oxysporum F3 genome again under the regulation of the gpdA promoter of Aspergillus nidulans. The transformation is effected through Agrobacterium tumefaciens. The transformants all show extracellular enzyme activity. Both transformants produce approximately 60% more ethanol compared to the wild type on corn cob, while for wheat bran this picture is repeated for only one of them, high extracellular xylanase activities in the transformants’ fermentation broths are maintained 2–2.5fold higher compared to the wild-type Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-
Fusarium oxysporum F3
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O
-
Fusarium oxysporum ?
-
?
birch wood xylan + H2O
-
Fusarium oxysporum F3 ?
-
?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-xylanase 2
-
Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Fusarium oxysporum

General Information

General Information Comment Organism
physiological function endo-beta-1,4-xylanase is one of the key enzymes of ethanol productivity during the consolidated bioprocessing of lignocellulosics Fusarium oxysporum