Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates at 1 mM | Alicyclobacillus sp. |
Cloned (Comment) | Organism |
---|---|
gene xynA4, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Alicyclobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Alicyclobacillus sp. | |
Hg2+ | - |
Alicyclobacillus sp. | |
Mn2+ | - |
Alicyclobacillus sp. | |
Ni2+ | - |
Alicyclobacillus sp. | |
SDS | - |
Alicyclobacillus sp. | |
Zn2+ | - |
Alicyclobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at 1 mM | Alicyclobacillus sp. | |
Co2+ | activates at 1 mM | Alicyclobacillus sp. | |
Cr3+ | activates at 1 mM | Alicyclobacillus sp. | |
Li+ | activates at 1 mM | Alicyclobacillus sp. | |
Mg2+ | activates at 1 mM | Alicyclobacillus sp. | |
additional information | the enzyme is unaffected by 1-10 mM K+, Na+, and EDTA | Alicyclobacillus sp. | |
Pb2+ | activates at 1 mM | Alicyclobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42500 | - |
x * 42500, about, sequence calculation | Alicyclobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus sp. | D3VWB5 | isolated from a hot spring in Baoshan City, Yunnan Province, China, gene xynA4 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Alicyclobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birch wood xylan + H2O | - |
Alicyclobacillus sp. | ? | - |
? | |
oat spelt xylan + H2O | - |
Alicyclobacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42500, about, sequence calculation | Alicyclobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase | - |
Alicyclobacillus sp. |
XynA4 | - |
Alicyclobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Alicyclobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | XynA4 shows over 50% of maximal activity within this range | Alicyclobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant XynA4, 1 h, pH 7.0, over 90% activity remaining, half-life is 394 min | Alicyclobacillus sp. |
65 | - |
purified recombinant XynA4, half-life is 17 min | Alicyclobacillus sp. |
70 | - |
purified recombinant XynA4, half-life is 3 min | Alicyclobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Alicyclobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.8 | 9.4 | XynA4 shows over 40% of maximal activity within this range | Alicyclobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.6 | 12 | purified recombinant XynA4, 1 h, 37°C, over 80% remaining activity | Alicyclobacillus sp. |