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Literature summary for 3.2.1.8 extracted from

  • Lafond, M.; Tauzin, A.; Desseaux, V.; Bonnin, E.; Ajandouz, E.; Giardina, T.
    GH10 xylanase D from Penicillium funiculosum: Biochemical studies and xylooligosaccharide production (2011), Microb. Cell Fact., 10, 20.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene xynD, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X33 Talaromyces funiculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, overview Talaromyces funiculosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Talaromyces funiculosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41540
-
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus
46000
-
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus
64000
-
x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus

Organism

Organism UniProt Comment Textmining
Talaromyces funiculosus Q5ZNB1 gene xynD
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant protein is N- and O-glycosylated Talaromyces funiculosus

Purification (Commentary)

Purification (Comment) Organism
recombinant XynD 1.9fold from Pichia pastoris strain X33 by ultrafiltration and gel filtration Talaromyces funiculosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
substrate specificity with different arabinoxylans, overview Talaromyces funiculosus
3 3.9 purified recombinant enzyme, pH 5.0, 70°C Talaromyces funiculosus
50.4
-
purified recombinant enzyme, substrate birchwood xylan, pH 5.0, 70°C Talaromyces funiculosus
115.9
-
purified recombinant enzyme, substrate high viscosity arabinoxylan, pH 5.0, 70°C Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O low to high viscosity arabinoxylans, and water-extractable wheat arabinoxylans with different ratio arabinose:xylose, overview Talaromyces funiculosus ?
-
?
beech wood xylan + H2O
-
Talaromyces funiculosus ?
-
?
birch wood xylan + H2O
-
Talaromyces funiculosus ?
-
?
additional information the catalytic efficiency increases slightly with increasing their chain length with a small difference of the XynD catalytic efficiency against the different xylooligosaccharides Talaromyces funiculosus ?
-
?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE, x * 46000, about, deglycoylated enzyme, mass spectrometry, x * 41540, sequence calculation Talaromyces funiculosus

Synonyms

Synonyms Comment Organism
endo-1,4-beta-xylanase
-
Talaromyces funiculosus
GH10 xylanase D
-
Talaromyces funiculosus
More XynD belongs to the GH10 family Talaromyces funiculosus
XynD
-
Talaromyces funiculosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Talaromyces funiculosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified recombinant enzyme, 180 min, no loss of activity Talaromyces funiculosus
80
-
purified recombinant enzyme, 10 min, loss of 80% activity Talaromyces funiculosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Talaromyces funiculosus

pI Value

Organism Comment pI Value Maximum pI Value
Talaromyces funiculosus isoelectric focusing
-
5.9

General Information

General Information Comment Organism
additional information XynD possesses four catalytic subsites with a high energy of interaction with the substrate and a fifth subsite with a small energy of interaction Talaromyces funiculosus