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Literature summary for 3.2.1.8 extracted from

  • Driss, D.; Bhiri, F.; Elleuch, L.; Bouly, N.; Stals, I.; Miled, N.; Blibech, M.; Ghorbel, R.; Chaabouni, S.
    Purification and properties of an extracellular acidophilic endo-1,4-beta-xylanase, naturally deleted in the thumb, from Penicillium occitanis Pol6 (2011), Process Biochem., 46, 1299-1306.
No PubMed abstract available
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits slightly at 5 mM Penicillium sp. 'occitanis'
Mg2+ inhibits slightly at 5 mM Penicillium sp. 'occitanis'

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium sp. 'occitanis'
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates at 5 mM Penicillium sp. 'occitanis'
Cu2+ activates at 5 mM Penicillium sp. 'occitanis'
Fe2+ activates at 5 mM Penicillium sp. 'occitanis'
Zn2+ activates at 5 mM Penicillium sp. 'occitanis'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21730
-
 x * 22000, Xyn3, SDS-PAGE, x * 21730, Xyn3, sequence calculation Penicillium sp. 'occitanis'
22000
-
 x * 22000, Xyn3, SDS-PAGE, x * 21730, Xyn3, sequence calculation Penicillium sp. 'occitanis'

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
-
-
Penicillium sp. 'occitanis' Pol6
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Xyn3 has a carbohydrate content of 10.83% Penicillium sp. 'occitanis'

Purification (Commentary)

Purification (Comment) Organism
native extracellular 5.6fold Xyn3 to homogeneity by anion exchange chromatography, concentration by PEG, and gel filtration Penicillium sp. 'occitanis'

Source Tissue

Source Tissue Comment Organism Textmining
additional information the filamentous fungus is grown on oat spelt xylan Penicillium sp. 'occitanis'
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
358
-
 purified enzyme, substrate oat spelt xylan, pH 3.0, 45°C Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oat spelt xylan + H2O PoXyn3 initially releases larger xylooligosaccharides from oat spelt xylan. These oligosaccharides are principally hydrolyzed to xylobiose and xylotriose Penicillium sp. 'occitanis' xylobiose + xylotriose + ?
-
 ?
oat spelt xylan + H2O PoXyn3 initially releases larger xylooligosaccharides from oat spelt xylan. These oligosaccharides are principally hydrolyzed to xylobiose and xylotriose Penicillium sp. 'occitanis' Pol6 xylobiose + xylotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 22000, Xyn3, SDS-PAGE, x * 21730, Xyn3, sequence calculation Penicillium sp. 'occitanis'
More Xyn3 homology modelling Penicillium sp. 'occitanis'

Synonyms

Synonyms Comment Organism
acidophilic endo-1,4-beta-xylanase
-
 Penicillium sp. 'occitanis'
Xyn3
-
 Penicillium sp. 'occitanis'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Penicillium sp. 'occitanis'

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 75% of the activity at 30°C, rapidly decrease in activity above 50°C Penicillium sp. 'occitanis'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified enzyme, 1 h, 91% activity remaining Penicillium sp. 'occitanis'
50
-
 purified enzyme, 1 h, 70% activity remaining Penicillium sp. 'occitanis'
60
-
 purified enzyme, 1 h, 50% activity remaining Penicillium sp. 'occitanis'
70
-
 purified enzyme, 15 min, 68% activity remaining Penicillium sp. 'occitanis'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Penicillium sp. 'occitanis'

pH Range

pH Minimum pH Maximum Comment Organism
2 9 activity range Penicillium sp. 'occitanis'

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10 the enzyme retains 100% of its activity over a wide pH range from 2.0 to 10.0 Penicillium sp. 'occitanis'