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Literature summary for 3.2.1.8 extracted from

  • Sukhumsirichart, W.; Deesukon, W.; Kawakami, T.; Matsumoto, S.; Seesom, W.; Sakamoto, T.
    Expression and characterization of recombinant GH11 xylanase from thermotolerant Streptomyces sp. SWU10 (2014), Appl. Biochem. Biotechnol., 172, 436-446.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 14% residual activity Streptomyces sp.
Hg2+ 1 mM, 3% residual activity Streptomyces sp.
Mn2+ 1 mM, 73% residual activity Streptomyces sp.
additional information not inhibitory: EDTA Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
birch wood xylan Km value is 2.3 mg/ml, pGH 6.0, 40°C Streptomyces sp.
additional information
-
wheat arabinoxylan Km value is 5.6 mg/ml, pGH 6.0, 40°C Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. F8WSY7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
32.6
-
pH 6.0, 40°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birch wood xylan + H2O
-
Streptomyces sp. xylobiose + xylotriose + xylotetraose + ? major products ?
wheat arabinoxylan + H2O
-
Streptomyces sp. ?
-
?

Subunits

Subunits Comment Organism
? x* 48000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
XynSW3
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
0.6 10.3
-
Streptomyces sp.