Application | Comment | Organism |
---|---|---|
synthesis | production of enzyme in Pichia pastoris with codon optimization. The activity of dual-copy enzyme is maximized at 15158 U/ml after 120 h of shaking | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 10 mM, 77% residual activity | Aspergillus niger | |
Fe2+ | 10 mM, 83% residual activity | Aspergillus niger | |
sodium dodecylsulfate | 10 mM, 72% residual activity | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
xylan | Km value is 18.27 mg/ml, pH 5.0, 50°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 10 mM, 119% of initial activity | Aspergillus niger | |
Tween 80 | 0.5%, 185% of initial activity | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | F5CI28 | - |
- |
Aspergillus niger IA-0010 | F5CI28 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6853 | - |
substrate birchwood xylan, pH 5.0, 50°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | almost no activity on xylobiose | Aspergillus niger | ? | - |
? | |
additional information | almost no activity on xylobiose | Aspergillus niger IA-0010 | ? | - |
? | |
xylan + H2O | substrate birchwood xylan | Aspergillus niger | xylobiose + D-xylose + ? | after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose | ? | |
xylan + H2O | substrate birchwood xylan | Aspergillus niger IA-0010 | xylobiose + D-xylose + ? | after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose | ? | |
xylohexaose + H2O | - |
Aspergillus niger | xylobiose + D-xylose + ? | - |
? | |
xylohexaose + H2O | - |
Aspergillus niger IA-0010 | xylobiose + D-xylose + ? | - |
? | |
xylopentaose + H2O | - |
Aspergillus niger | xylobiose + D-xylose | - |
? | |
xylotetraose + H2O | - |
Aspergillus niger | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus niger IA-0010 | 2 xylobiose | - |
? | |
xylotriose + H2O | - |
Aspergillus niger | xylobiose + D-xylose | - |
? | |
xylotriose + H2O | - |
Aspergillus niger IA-0010 | xylobiose + D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
XynB | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, complete loss of activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |