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Literature summary for 3.2.1.8 extracted from
- Purification, biochemical characterization, and structure of recombinant endo-1,4-beta-xylanase XylE (2012), Biochemistry (Moscow), 77, 1190-1198.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Penicillium canescens | Penicillium canescens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 1.47 A reslution. The catalytic residues are Glu158 and Glu270, in a distance of 4.98 A. At a distance of 2.58 A from Glu158, the residue Glu238 is located | Penicillium canescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
birch wood xylan | Km value is 0.52 mg/ml, pH 5.0, 50°C | Penicillium canescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Penicillium canescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium canescens | C3VEV9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Penicillium canescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birch wood xylan + H2O | - |
Penicillium canescens | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Penicillium canescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XylE | - |
Penicillium canescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Penicillium canescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 75 | more than 80% of maximum activity | Penicillium canescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life 7 h | Penicillium canescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Penicillium canescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Penicillium canescens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Penicillium canescens | isoelectric focusing | - |
6.5 |
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