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Literature summary for 3.2.1.8 extracted from
- Cloning, over-expression and characterization of a thermo-tolerant xylanase from Thermotoga thermarum (2014), Biotechnol. Lett., 36, 587-593.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudothermotoga thermarum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| homology modeling of structure. Residues Glu145 and Glu252 are the catalytic nucleophile and proton donor, respectively | Pseudothermotoga thermarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 1 mM, 46% residual activity | Pseudothermotoga thermarum |  |
| Ba2+ | 1 mM, 72% residual activity | Pseudothermotoga thermarum | |
| Cu2+ | 1 mM, no residual activity | Pseudothermotoga thermarum | |
| Mg2+ | 1 mM, 46% residual activity | Pseudothermotoga thermarum | |
| sodium dodecylsulfate | 0.1%, 82% residual activity | Pseudothermotoga thermarum | |
| Tween 60 | 0.05%, 78% residual activity | Pseudothermotoga thermarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beechwood xylan | Km value is 1.8 mg/ml, pH 6.0, 80°C | Pseudothermotoga thermarum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 1 mM, 133% of initial activity | Pseudothermotoga thermarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40531, calculated, x * 40000, SDS-PAGE | Pseudothermotoga thermarum |
| 40531 | - |
x * 40531, calculated, x * 40000, SDS-PAGE | Pseudothermotoga thermarum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudothermotoga thermarum | F7YXD6 | - |
- |
| Pseudothermotoga thermarum DSM 5069 | F7YXD6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Pseudothermotoga thermarum | ? | - |
? | |
| beechwood xylan + H2O | - |
Pseudothermotoga thermarum DSM 5069 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40531, calculated, x * 40000, SDS-PAGE | Pseudothermotoga thermarum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Xyn10B | - |
Pseudothermotoga thermarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Pseudothermotoga thermarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
2 h, 75% resiudal activity | Pseudothermotoga thermarum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 520 | - |
beechwood xylan | pH 6.0, 80°C | Pseudothermotoga thermarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Pseudothermotoga thermarum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | 50°C, stable for 1 h | Pseudothermotoga thermarum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudothermotoga thermarum | calculated | - |
6.4 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beechwood xylan | kcat/Km value is 289 ml per mg and s, pH 6.0, 80°C | Pseudothermotoga thermarum |
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