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Literature summary for 3.2.1.8 extracted from

  • Shrivastava, S.; Shukla, P.; Deepalakshmi, P.D.; Mukhopadhyay, K.
    Characterization, cloning and functional expression of novel xylanase from Thermomyces lanuginosus SS-8 isolated from self-heating plant wreckage material (2013), World J. Microbiol. Biotechnol., 29, 2407-2415.
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
degradation enzyme is able to degrade pulp and release substantial chromophoric materials and lignin derived compounds indicating its effective utility in pulp bleaching Thermomyces lanuginosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermomyces lanuginosus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Thermomyces lanuginosus
Fe2+ strong inhibition Thermomyces lanuginosus
Hg2+ strong inhibition Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 oat spelt xylan Km value is 10.9 mg/ml, pH 6.0, 60°C Thermomyces lanuginosus
additional information
-
 beechwood xylan Km value is 17.7 mg/ml, pH 6.0, 60°C Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21300
-
 x * 21300, SDS-PAGE Thermomyces lanuginosus
23800
-
 PAGE Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus O43097
-
-
Thermomyces lanuginosus SS-8 O43097
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Thermomyces lanuginosus xylose + ?
-
 ?
beechwood xylan + H2O
-
 Thermomyces lanuginosus SS-8 xylose + ?
-
 ?
oat spelt xylan + H2O
-
 Thermomyces lanuginosus xylose + ? no release of xylo-oligosaccharides detected ?
oat spelt xylan + H2O
-
 Thermomyces lanuginosus SS-8 xylose + ? no release of xylo-oligosaccharides detected ?

Subunits

Subunits Comment Organism
? x * 21300, SDS-PAGE Thermomyces lanuginosus

Synonyms

Synonyms Comment Organism
XynA
-
 Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 96 h, 80% residual activity Thermomyces lanuginosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2600
-
 oat spelt xylan pH 6.0, 60°C Thermomyces lanuginosus
3430
-
 beechwood xylan pH 6.0, 60°C Thermomyces lanuginosus

pH Range

pH Minimum pH Maximum Comment Organism
3 11
-
 Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus isoelectric focusing
-
 3.9