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Literature summary for 3.2.1.8 extracted from

  • Oh, H.W.; Heo, S.Y.; Kim, D.Y.; Park, D.S.; Bae, K.S.; Park, H.Y.
    Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12 (2008), Antonie Van Leeuwenhoek, 93, 437-42.
    View publication on PubMed
Search for more literature for 3.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, more than 80% inhibition Cellulosimicrobium sp.
Co2+ 10 mM, more than 80% inhibition Cellulosimicrobium sp.
Cu2+ 10 mM, more than 80% inhibition Cellulosimicrobium sp.
Fe2+ 10 mM, more than 80% inhibition Cellulosimicrobium sp.
additional information not inhibitory: Mg2+, Mn2+ Cellulosimicrobium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 10 mM, 30% increase in activity Cellulosimicrobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
-
 Cellulosimicrobium sp.

Organism

Organism UniProt Comment Textmining
Cellulosimicrobium sp. B2BZ80
-
-
Cellulosimicrobium sp. HY-12 B2BZ80
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 substrate 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 50°C Cellulosimicrobium sp.
2.1
-
 substrate 4-nitrophenyl beta-D-cellobioside, pH 6.0, 50°C Cellulosimicrobium sp.
14.9
-
 substrate oat spelt xylan, pH 6.0, 50°C Cellulosimicrobium sp.
19.2
-
 substrate birchwood xylan, pH 6.0, 50°C Cellulosimicrobium sp.
21.2
-
 substrate beechwood xylan, pH 6.0, 50°C Cellulosimicrobium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
 Cellulosimicrobium sp. 4-nitrophenol + beta-D-cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Cellulosimicrobium sp. HY-12 4-nitrophenol + beta-D-cellobiose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellulosimicrobium sp. 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Cellulosimicrobium sp. HY-12 4-nitrophenol + beta-D-xylopyranose
-
 ?
beechwood xylan + H2O
-
 Cellulosimicrobium sp. xylo-oligosaccharides + ?
-
 ?
beechwood xylan + H2O
-
 Cellulosimicrobium sp. HY-12 xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O
-
 Cellulosimicrobium sp. xylo-oligosaccharides + ?
-
 ?
birchwood xylan + H2O
-
 Cellulosimicrobium sp. HY-12 xylo-oligosaccharides + ?
-
 ?
additional information no substrates: carboxymethyl cellulose or xylobiose Cellulosimicrobium sp. ?
-
 ?
additional information no substrates: carboxymethyl cellulose or xylobiose Cellulosimicrobium sp. HY-12 ?
-
 ?
oat spelt xylan + H2O
-
 Cellulosimicrobium sp. xylo-oligosaccharides + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE, x * 42925, calculated Cellulosimicrobium sp.

Synonyms

Synonyms Comment Organism
XYLA
-
 Cellulosimicrobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Cellulosimicrobium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable below for at least 1 h Cellulosimicrobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Cellulosimicrobium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 more than 80% of maximum activity Cellulosimicrobium sp.