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Literature summary for 3.2.1.8 extracted from

  • Kim, D.Y.; Lee, S.H.; Lee, M.J.; Cho, H.Y.; Lee, J.S.; Rhee, Y.H.; Shin, D.H.; Son, K.H.; Park, H.Y.
    Genetic and functional characterization of a novel GH10 endo-beta-1,4-xylanase with a ricin-type beta-trefoil domain-like domain from Luteimicrobium xylanilyticum HY-24 (2018), Int. J. Biol. Macromol., 106, 620-628 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 0.5%, about 120% of initial activity Luteimicrobium xylanilyticum
Tween 80 0.5%, about 120% of initial activity Luteimicrobium xylanilyticum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Luteimicrobium xylanilyticum

Protein Variants

Protein Variants Comment Organism
additional information construction of a deletion mutant lacking the C-terminal ricin-type beta-trefoil lectin domain-like part. Mutant shows a decrease in binding capacities to lignin and insoluble polysaccharides Luteimicrobium xylanilyticum

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, complete loss of activity Luteimicrobium xylanilyticum
additional information stimulation or inhibition by 1 mM of Ca2+,Ni2+, Zn2+, Mg2+, Mn2+, Sn2+, Ba2+, Co2+, and Fe2+ is marginal Luteimicrobium xylanilyticum
N-bromosuccinimide 5 mM, complete loss of activity Luteimicrobium xylanilyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 oat spelt xylan Km value 1.3 mg/ml, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 beechwood xylan Km value 1.4 mg/ml, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 birchwood xylan Km value 1.5 mg/ml, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 wheat arabinoxylan Km value 2.8 mg/ml, pH 6.0, 65°C Luteimicrobium xylanilyticum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information stimulation or inhibition by 1 mM of Ca2+,Ni2+, Zn2+, Mg2+, Mn2+, Sn2+, Ba2+, Co2+, and Fe2+ is marginal Luteimicrobium xylanilyticum

Organism

Organism UniProt Comment Textmining
Luteimicrobium xylanilyticum A0A286LNP2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
74.8
-
 substrate oat spelt xylan, C-terminal truncated mutant, pH 6.0, 65°C Luteimicrobium xylanilyticum
254
-
 substrate oat spelt xylan, wild-type, pH 6.0, 65°C Luteimicrobium xylanilyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
 Luteimicrobium xylanilyticum ?
-
 ?
birchwood xylan + H2O
-
 Luteimicrobium xylanilyticum xylotetraose + xylotriose + xylobiose + xylose hydrolysis product composition is 1.4% xylose, 37.1% xylobiose, 58.9% xylotriose and 2.6% xylotetraose ?
additional information activity decreases in the following order: oat spelt xylan, beechwood xylan, birchwood xylan, wheat arabinoxylan. No substrates: xyloglucan, carboxymethylcellulose, locust beangum, and 4-nitrophenyl sugar derivatives Luteimicrobium xylanilyticum ?
-
 ?
oat spelt xylan + H2O
-
 Luteimicrobium xylanilyticum ?
-
 ?
wheat arabinoxylan + H2O
-
 Luteimicrobium xylanilyticum ?
-
 ?
xylohexaose + H2O
-
 Luteimicrobium xylanilyticum xylopentaose + xylotetraose + xylotriose + xylobiose + xylose hydrolysis product composition is 1.2% xylose, 29.8% xylobiose, 56.4% xylotriose, 10.9% xylotetraose and 1.7% xylopentaose ?
xylopentaose + H2O
-
 Luteimicrobium xylanilyticum xylopentaose + xylotetraose + xylotriose + xylobiose + xylose hydrolysis product composition is 1.2% xylose, 38.4% xylobiose, 49.1% xylotriose, 6.7% xylotetraose and 4.6% xylopentaose ?
xylotetraose + H2O
-
 Luteimicrobium xylanilyticum xylotetraose + xylotriose + xylobiose + xylose hydrolysis product composition is 1.9% xylose, 34.9% xylobiose, 49.2% xylotriose and 14.0% xylotetraose ?

Subunits

Subunits Comment Organism
? x * 49000, SDS-PAGE, recombinant protein, x * 50480, calculated Luteimicrobium xylanilyticum

Synonyms

Synonyms Comment Organism
xylM
-
 Luteimicrobium xylanilyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 C-terminal truncated mutant Luteimicrobium xylanilyticum
65
-
 wild-type Luteimicrobium xylanilyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 C-terminal truncated mutant, loss of activity above 40°C Luteimicrobium xylanilyticum
60
-
 1 h, 80% residual activity Luteimicrobium xylanilyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
85
-
 wheat arabinoxylan pH 6.0, 65°C Luteimicrobium xylanilyticum
222
-
 birchwood xylan pH 6.0, 65°C Luteimicrobium xylanilyticum
248
-
 beechwood xylan pH 6.0, 65°C Luteimicrobium xylanilyticum
390
-
 oat spelt xylan pH 6.0, 65°C Luteimicrobium xylanilyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 C-terminal truncated mutant Luteimicrobium xylanilyticum
6
-
 wild-type Luteimicrobium xylanilyticum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 birchwood xylan kcat/Km value 153 ml/mg/s, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 beechwood xylan kcat/Km value 178 ml/mg/s, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 wheat arabinoxylan kcat/Km value 30 ml/mg/s, pH 6.0, 65°C Luteimicrobium xylanilyticum
additional information
-
 oat spelt xylan kcat/Km value 309 ml/mg/s, pH 6.0, 65°C Luteimicrobium xylanilyticum