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Literature summary for 3.2.1.8 extracted from

  • Rahmani, N.; Kahar, P.; Lisdiyanti, P.; Lee, J.; Yopi, J.; Prasetya, B.; Ogino, C.; Kondo, A.
    GH-10 and GH-11 endo-1,4-beta-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition (2019), Biores. Technol., 272, 315-325 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene Xyl10_1479, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Streptomyces lividans strain 1326, the enzyme is secreted Kitasatospora sp.
gene Xyl11_1020, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Streptomyces lividans strain 1326, the enzyme is secreted Kitasatospora sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by xylose; no inhibition by xylose Kitasatospora sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Kitasatospora sp. hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges ?
-
 ?
additional information Kitasatospora sp. XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges ?
-
 ?
additional information Kitasatospora sp. BTCC B-807 hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges ?
-
 ?
additional information Kitasatospora sp. BTCC B-807 XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges ?
-
 ?

Organism

Organism UniProt Comment Textmining
Kitasatospora sp. A0A3T0YV60
-
-
Kitasatospora sp. A0A3T0YV67
-
-
Kitasatospora sp. BTCC B-807 A0A3T0YV60
-
-
Kitasatospora sp. BTCC B-807 A0A3T0YV67
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cultured on sugarcane bagasse Kitasatospora sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O best substrate Kitasatospora sp. ?
-
 ?
birchwood xylan + H2O 50% activity compared to beechwood xylan Kitasatospora sp. ?
-
 ?
additional information hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges Kitasatospora sp. ?
-
 ?
additional information XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges Kitasatospora sp. ?
-
 ?
additional information hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges. No activityy with carboxymethylcellulose, soluble starch, and Avicel. Substrate specificity, overview Kitasatospora sp. ?
-
 ?
additional information XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges. No activityy with carboxymethylcellulose, soluble starch, and Avicel. Substrate specificity, overview Kitasatospora sp. ?
-
 ?
additional information hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges Kitasatospora sp. BTCC B-807 ?
-
 ?
additional information hydrolysis of xylan, extracted of bagasse, by XYN11Ks_480 produces xylooligosaccharides without xylose formation. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-1,4-xylosidic bridges. No activityy with carboxymethylcellulose, soluble starch, and Avicel. Substrate specificity, overview Kitasatospora sp. BTCC B-807 ?
-
 ?
additional information XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges Kitasatospora sp. BTCC B-807 ?
-
 ?
additional information XYN10Ks_480 shows high hydrolytic activity with xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides. Xylose sugar oligomers contain 2-7 xylose units (X2-X7) linked by beta-(1,4)-xylosidic bridges. No activityy with carboxymethylcellulose, soluble starch, and Avicel. Substrate specificity, overview Kitasatospora sp. BTCC B-807 ?
-
 ?
oat spelt xylan + H2O 50% activity compared to beechwood xylan Kitasatospora sp. ?
-
 ?
xylobiose + H2O
-
 Kitasatospora sp. 2 D-xylose
-
 ?
xylobiose + H2O
-
 Kitasatospora sp. BTCC B-807 2 D-xylose
-
 ?
xylohexaose + H2O
-
 Kitasatospora sp. xylose + ?
-
 ?
xylohexaose + H2O
-
 Kitasatospora sp. BTCC B-807 xylose + ?
-
 ?
xylopentaose + H2O
-
 Kitasatospora sp. xylose + ?
-
 ?
xylopentaose + H2O
-
 Kitasatospora sp. BTCC B-807 xylose + ?
-
 ?
xylotetraose + 3 H2O
-
 Kitasatospora sp. 4 xylose
-
 ?
xylotriose + 2 H2O
-
 Kitasatospora sp. 3 xylose
-
 ?

Subunits

Subunits Comment Organism
More tertiary structural homology modeling and the three-dimensional structure of the xylanase, overview Kitasatospora sp.
More the enzyme possesses an N-terminal catalytic glycosyl hydrolase family 11 (GH-11) domain and and a C-terminal CBM 2 domain, tertiary structural homology modeling and the three-dimensional structure of the xylanase, overview. The XYN11Ks_480 endo-1,4-beta-xylanase has a compact globular structure with a single alpha-helix and two extended pleated beta-sheets that formed a jelly-roll fold. The main feature is the presence of a long cleft that spanned the entire molecule. This cleft contains a site of activity that included two glutamate residues that directly participate in xylan hydrolysis wherein one acts as an acid/base (position 165) catalyst and the other acts as a nucleophile (position 273) Kitasatospora sp.

Synonyms

Synonyms Comment Organism
GH-10 endo-1,4-beta-xylanase
-
 Kitasatospora sp.
GH-11 endo-1,4-beta-xylanase
-
 Kitasatospora sp.
XYN10Ks_480
-
 Kitasatospora sp.
XYN11Ks_480
-
 Kitasatospora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Kitasatospora sp.
55
-
-
 Kitasatospora sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 50% of maximal activity within this range, profile overview Kitasatospora sp.
30 65 50% of maximal activity within this range, profile overview Kitasatospora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 purified recombinant enzyme, pH 5.0, stable for 120 min Kitasatospora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Kitasatospora sp.

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 50% of maximal activity within this range, acetate and citrate buffer, profile overview Kitasatospora sp.
4.5 6.5 50% of maximal activity within this range, acetate and phosphate buffer, profile overview Kitasatospora sp.

General Information

General Information Comment Organism
evolution the enzyme belongs to the CBM2 superfamily, glycoside hydrolase family 10, GH10 Kitasatospora sp.
evolution the enzyme belongs to the CBM2 superfamily, glycoside hydrolase family 11, GH11 Kitasatospora sp.
additional information XYN10Ks_480 is an endo-1,4-beta-xylanase with a ricin-type beta-trefoil type of domain, tertiary structural homology modeling and the three-dimensional structure of the xylanase, overview Kitasatospora sp.
additional information XYN11Ks_480 is an endo-1,4-beta-xylanase with an N-terminal catalytic glycosyl hydrolase family 11 (GH-11) domain and and a C-terminal CBM 2 domain, tertiary structural homology modeling and the three-dimensional structure of the xylanase, overview. The XYN11Ks_480 endo-1,4-beta-xylanase has a compact globular structure with a single alpha-helix and two extended pleated beta-sheets that formed a jelly-roll fold. The main feature is the presence of a long cleft that spanned the entire molecule. This cleft contains a site of activity that included two glutamate residues that directly participate in xylan hydrolysis wherein one acts as an acid/base (psoition 165) catalyst and the other acts as a nucleophile (position 273) Kitasatospora sp.
physiological function high levels of expression and catalytic activity for XYN10Ks_480 during hydrolysis of xylan extracted of bagasse, three types of xylan-based substrates are used to produce xylose and xylooligosaccharides Kitasatospora sp.