General Stability | Organism |
---|---|
the immobilized enzyme retains more than 70% of its initial capability for xylooligosaccharide production after five reaction cycles | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | A0A172MAU1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | the hydrolysis products are mainly xylobiose (57.5% with immobilized enzyme, 36.1% with soluble enzyme) and xylotriose (38.4% with immobilized enzyme, 48.7% with soluble enzyme) | Bacillus subtilis | xylobiose + xylotriose + xylotetraose | - |
? | |
xylooligosaccharides + H2O | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase LC9 | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
immobilized enzyme and soluble enzyme | Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: immobilized enzyme shows about 50% of maximal activity, soluble enzyme shows about 40% of maximal activity. 70°C: immobilized enzyme shows about 65% of maximal activity, soluble enzyme shows about 50% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
3 h, immobilized enzyme retains 45.3% of its activity, soluble enzyme retains 16.3% of its activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
immobilized enzyme and soluble enzyme | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
additional information | a one-step method to immobilize xylanase onto cellulosic material by fusion of expansin from Bacillus subtilis to xylanase LC9 without the requirement of prior purification of enzyme is developed. Fusion enzyme EXLXR2-XYN is specifically adsorbed onto corncob residue with high loading capacity due to bio-affinity adsorption of expansin onto cellulose. The immobilization yield is close to 100%, with a recovered activity of 82.4% | Bacillus subtilis |