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Literature summary for 3.2.1.8 extracted from

  • Ouephanit, C.; Boonvitthya, N.; Theerachat, M.; Bozonnet, S.; Chulalaksananukul, W.
    Efficient expression and secretion of endo-1,4-beta-xylanase from Penicillium citrinum in non-conventional yeast Yarrowia lipolytica directed by the native and the preproLIP2 signal peptides (2019), Protein Expr. Purif., 160, 1-6 .
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA, DNA and amino acid sequence determination and analysis, recombinant expression in Yarrowia lipolytica under control of the strong constitutive TEF promoter with the preproLIP2 secretion signal using plasmid JMP62_TEF_Ura3Ex, the enzyme is secreted. Recombinant expression in Pichia pastoris strain GS115 results in only low enzyme activity Penicillium citrinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km of the recombinant enzyme for birchwood xylan is 5.2 mg/ml, pH 5.0, 55°C Penicillium citrinum

Organism

Organism UniProt Comment Textmining
Penicillium citrinum Q2PGY1
-
-
Penicillium citrinum FERM P-15944 Q2PGY1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant extrecellular enzyme is highly glycosylated Penicillium citrinum

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 185fold, the enzyme is deglycosylated by EndoH, followed by ultrafiltration, and anion exchange chromatography Penicillium citrinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
695.2
-
 purified recombinant deglycosylated extracellular enzyme, pH 5.0, 55°C, substrate birchwood xylan Penicillium citrinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azo-xylan + H2O
-
 Penicillium citrinum ?
-
 ?
azo-xylan + H2O
-
 Penicillium citrinum FERM P-15944 ?
-
 ?
birchwood xylan + H2O
-
 Penicillium citrinum xylobiose + xylotriose + ?
-
 ?
birchwood xylan + H2O
-
 Penicillium citrinum FERM P-15944 xylobiose + xylotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, recombinant extracellular deglycosylated enzyme, SDS-PAGE, x * 60000, above, recombinant extracellular glycosylated enzyme, SDS-PAGE Penicillium citrinum

Synonyms

Synonyms Comment Organism
xylanase A
-
 Penicillium citrinum
XynA
-
 Penicillium citrinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 recombinant enzyme Penicillium citrinum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 recombinant enzyme, over 50% of maximal activity within this range, profile overview Penicillium citrinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified recombinant extracellular deglycosylated enzyme, 6 h, over 80% activity remains Penicillium citrinum
45
-
 purified recombinant extracellular deglycosylated enzyme, 70% activity remains after 30 min, 17% after 3 h, and 6% after 5 h Penicillium citrinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
245
-
 birchwood xylan recombinant enzyme, pH 5.0, 55°C Penicillium citrinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant enzyme Penicillium citrinum

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.5 recombinant enzyme, over 50% of maximal activity within this range, profile overview Penicillium citrinum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 purified recombinant extracellular deglycosylated enzyme, 24 h, over 80% activity remains Penicillium citrinum