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Literature summary for 3.2.1.80 extracted from

  • Gill, P.K.; Manhas, R.K.; Singh, J.; Singh, P.
    Purification and characterization of an exoinulinase from Aspergillus fumigatus (2004), Appl. Biochem. Biotechnol., 117, 19-32.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 63% of inhibition Aspergillus fumigatus
Hg2+ 1 mM, 100% of inhibition Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
inulin
-
Aspergillus fumigatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus fumigatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59400
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
62700
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
66000
-
1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus
176500
-
native PAGE Aspergillus fumigatus
200000
-
gel filtration Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ammonium sulfate precipitation, DEAE-Sephacel column, Sephacryl S-200 column, concanavalin A-linked amino-activated silica column and Sepharose 6B column, 25fold Aspergillus fumigatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
171 U/mg of protein Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
Aspergillus fumigatus oligofructosides
-
?
raffinose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
?
stachyose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
-
?
sucrose + H2O
-
Aspergillus fumigatus beta-D-fructose + alpha-D-glucose
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE Aspergillus fumigatus

Synonyms

Synonyms Comment Organism
exoinulinase
-
Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
enzyme retains 35.9% and 25.8% activities after 4 h at 50 and 55ºC, respectively Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Aspergillus fumigatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5
-
Aspergillus fumigatus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus fumigatus isoelectric focusing 4.7 4.6