Literature summary for 3.2.1.80 extracted from

Kulminskaya, A.A.; Arand, M.; Eneyskaya, E.V.; Ivanen, D.R.; Shabalin, K.A.; Shishlyannikov, S.M.; Saveliev, A.N.; Korneeva, O.S.; Neustroev, K.N.
Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysis (2003), Biochim. Biophys. Acta, 1650, 22-29.

Search for more literature for 3.2.1.80

Application

Application	Comment	Organism
nutrition	microbial production of pure beta-D-fructofuranose or fructose syrup	Aspergillus awamori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Aspergillus awamori
0.05	-	inulin	at 37ºC, pH 4.5	Aspergillus awamori
0.21	-	levanotriose	at 37ºC, pH 4.5	Aspergillus awamori
0.94	-	inulotetraose	at 37ºC, pH 4.5	Aspergillus awamori
1.01	-	levanohexaose	at 37ºC, pH 4.5	Aspergillus awamori
1.02	-	inulohexaose	at 37ºC, pH 4.5	Aspergillus awamori
1.08	-	inulopentaose	at 37ºC, pH 4.5	Aspergillus awamori
1.1	-	inuloheptaose	at 37ºC, pH 4.5	Aspergillus awamori
1.63	-	inulotriose	at 37ºC, pH 4.5	Aspergillus awamori
2.74	-	levanotetraose	at 37ºC, pH 4.5	Aspergillus awamori
3.72	-	Inulobiose	at 37ºC, pH 4.5	Aspergillus awamori
10.2	-	levanopentaose	at 37ºC, pH 4.5	Aspergillus awamori
40	-	sucrose	at 37ºC, pH 4.5	Aspergillus awamori

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus awamori	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
inulin + 5 H2O	Aspergillus awamori	-	5 beta-D-fructofuranose + alpha-D-glucopyranose	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus awamori	Q96TU3	var. 2250	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no modification	-	Aspergillus awamori

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture medium	-	Aspergillus awamori	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
inulin + 5 H2O	-	Aspergillus awamori	5 beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inulobiose + H2O	inulin is an intermediate product	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inuloheptaose + H2O	inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inulohexaose + H2O	inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inulopentaose + H2O	inulotetraose, inulotriose, inulobiose and inulin are intermediate products	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inulotetraose + H2O	inulotriose, inulobiose and inulin are intermediate products	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
inulotriose + H2O	inulobiose and inulin are intermediate products	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?
levan + H2O	-	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose + ?	-	?
levanohexaose + 6 H2O	levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products	Aspergillus awamori	6 beta-D-fructofuranose	-	?
levanopentaose + 4 H2O	levanotetraose, levanotriose, levanobiose and levan are intermediate products	Aspergillus awamori	5 beta-D-fructofuranose	-	?
levanotetraose + 3 H2O	levanotriose, levanobiose and levan are intermediate products	Aspergillus awamori	4 beta-D-fructofuranose	-	?
levanotriose + 2 H2O	levanobiose and levan are intermediate products	Aspergillus awamori	3 beta-D-fructofuranose	-	?
sucrose + H2O	-	Aspergillus awamori	beta-D-fructofuranose + alpha-D-glucopyranose	-	?

Synonyms

Synonyms	Comment	Organism
2,1-beta-D-fructan fructohydrolase	-	Aspergillus awamori
exoinulinase	-	Aspergillus awamori

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Release 2023.1 (January 2023)