Cloned (Comment) | Organism |
---|---|
- |
Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
1-kestose | pH 5.0, 30°C | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
1 * 70000, SDS-PAGE | Triticum aestivum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Triticum aestivum | the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
winter wheat | - |
Purification (Comment) | Organism |
---|---|
two isoforms 1-FEH w1 and w2 | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | comparison of activities during stem development | Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.2 | - |
isoform 1-FEH w1 | Triticum aestivum |
14.6 | - |
isoform 1-FEH w1 | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,1-nystose + H2O | 72% activity of isoform w1 and 83% of w2 in comparison to substrate 1-kestose | Triticum aestivum | beta-D-fructose + sucrose | - |
? | |
1-kestose + H2O | 100% activity of both isoforms w1 and w2 | Triticum aestivum | beta D-fructofuranose + sucrose | - |
? | |
6-kestose + H2O | 0.7% activity of isoform w1 and 1% of w2 in comparison to substrate 1-kestose | Triticum aestivum | beta-D-fructose + sucrose | - |
? | |
inulin + H2O | 39% activity of isoform w1 and 22% of w2 in comparison to substrate 1-kestose | Triticum aestivum | D-fructose + ? | - |
? | |
levan + H2O | 4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose | Triticum aestivum | D-fructose + ? | - |
? | |
additional information | the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan | Triticum aestivum | ? | - |
? | |
neokestose + H2O | 4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose | Triticum aestivum | beta-D-fructose + sucrose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
fructan 1-exohydrolase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | both isoforms w1 and w2 | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | both isoforms w1 and w2 | Triticum aestivum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
above pH 7.5 the isoforms are inactive | Triticum aestivum |