Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Cichorium intybus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method, crystallization of complexes between 1-FEH IIa with the substrate 1-kestose and the inhibitors sucrose, fructose and 2,5-dideoxy-2,5-imino-D-mannitol | Cichorium intybus |
mutant proteins are crystallized by hanging drop vapour diffusion method with 2.1 M sodium potassium phosphate and 0.1 M potassium phosphate, pH 7.0 | Cichorium intybus |
Protein Variants | Comment | Organism |
---|---|---|
E201Q | inactive | Cichorium intybus |
S101L | inactive | Cichorium intybus |
S101L | no inhibition by sucrose | Cichorium intybus |
W82L | inactive | Cichorium intybus |
W82L | no inhibition by sucrose | Cichorium intybus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,5 dideoxy-2,5-imino-D-mannitol | - |
Cichorium intybus | |
D-fructose | - |
Cichorium intybus | |
sucrose | 63% inhibition by 10 mM sucrose, wild-type enzyme. Mutant enzymes W82L and S101L are not inhibited by sucrose. W82 and S101 are important for binding sucrose as inhibitor | Cichorium intybus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium intybus | Q93X60 | precursor | - |
Purification (Comment) | Organism |
---|---|
- |
Cichorium intybus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | - |
Cichorium intybus | ? | - |
? | |
1-kestose + H2O | - |
Cichorium intybus | beta-D-fructose + sucrose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-FEH IIa | - |
Cichorium intybus |
fructan 1-exohydrolase | - |
Cichorium intybus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
sucrose | - |
Cichorium intybus |