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Literature summary for 3.2.1.80 extracted from
- Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.) (2011), Chem. Cent. J., 5, 16.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Arctium lappa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65666 | - |
x * 65666, calculated from amino acid sequence | Arctium lappa |
| 80000 | - |
x * 80000, SDS-PAGE | Arctium lappa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arctium lappa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose CL-6B column chromatography, Toyopearl HW55S column chromatography, and Toyopearl HW55S column chromatography | Arctium lappa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.765 | - |
enzyme from supernatant, at pH 6.5 and 30°C | Arctium lappa |
| 73.4 | - |
after 41.6fold purification, at pH 6.5 and 30°C | Arctium lappa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + H2O | 100% activity | Arctium lappa | ? | - |
? |  |
| 6-kestose + H2O | 2% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| burdock root sugar extract + H2O | - |
Arctium lappa | fructose + sucrose + ? | - |
? | |
| fructosylnystose + H2O | 97% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| inulin + H2O | 42% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| inulobiose + H2O | 12% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| inulotriose + H2O | 41% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| additional information | no activity towards levan | Arctium lappa | ? | - |
? | |
| additional information | the enzyme preferentially hydrolyzes the beta-2,1 linkage of terminal fructosyl residue of fructan | Arctium lappa | ? | - |
? | |
| neokestose + H2O | 7% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| nystose + H2O | 66% activity compared to 1-kestose | Arctium lappa | ? | - |
? | |
| sucrose + H2O | 0.2% activity compared to 1-kestose | Arctium lappa | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Arctium lappa |
| ? | x * 65666, calculated from amino acid sequence | Arctium lappa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-FEH | - |
Arctium lappa |
| fructan 1-exohydrolase | - |
Arctium lappa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable at temperatures up to 40°C | Arctium lappa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Arctium lappa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Arctium lappa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Arctium lappa | calculated from amino acid sequence | - |
4.9 |
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