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Literature summary for 3.2.1.80 extracted from

  • Xu, H.; Liang, M.; Xu, L.; Li, H.; Zhang, X.; Kang, J.; Zhao, Q.; Zhao, H.
    Cloning and functional characterization of two abiotic stress-responsive Jerusalem artichoke (Helianthus tuberosus) fructan 1-exohydrolases (1-FEHs) (2015), Plant Mol. Biol., 87, 81-98 .
    View publication on PubMed
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Helianthus tuberosus

Inhibitors

Inhibitors Comment Organism Structure
sucrose 13% inhibition of isoform 1-FEH I at 10 mM and 49% inhibition at 100 mM sucrose; 21% inhibition of isoform 1-FEH II at 10 mM and 65% at 100 mM sucrose Helianthus tuberosus

Organism

Organism UniProt Comment Textmining
Helianthus tuberosus A0A0D5A4E4 Jerusalem artichoke
-
Helianthus tuberosus A0A0D5A4L3 Jerusalem artichoke
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein isoform 1-FEH I has seven potential N-glycosylation sites Helianthus tuberosus
glycoprotein isoform 1-FEH II has four potential N-glycosylation sites Helianthus tuberosus

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Ni-NTA column chromatography Helianthus tuberosus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Helianthus tuberosus
-
stem
-
 Helianthus tuberosus
-
tuber
-
 Helianthus tuberosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O 100% activity Helianthus tuberosus ?
-
 ?
1F-fructofuranosylnystose + H2O 98% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
1F-fructofuranosylnystose + H2O 99% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
inulin + H2O 25% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
inulin + H2O 31% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
levan + H2O 2% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
levan + H2O 0.9% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
additional information inactive with sucrose Helianthus tuberosus ?
-
 ?
nystose + H2O 52% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
nystose + H2O 58% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?
sucrose + H2O 0.3% activity compared to 1-kestose Helianthus tuberosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62800, isoform 1-FEH I, calculated from amino acid sequence Helianthus tuberosus
? x * 65100, isoform 1-FEH II, calculated from amino acid sequence Helianthus tuberosus
? x * 90000, isoform 1-FEH I, SDS-PAGE Helianthus tuberosus
? x * 90000, isoform 1-FEH II, SDS-PAGE Helianthus tuberosus

Synonyms

Synonyms Comment Organism
1-FEH I isoform Helianthus tuberosus
1-FEH II isoform Helianthus tuberosus
fructan 1-exohydrolase
-
 Helianthus tuberosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Helianthus tuberosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 isoform 1-FEH I shows more than 70% activity between 25 and 40°C Helianthus tuberosus
30 40 isoform 1-FEH II shows about 40% activity at 30°C, 100% activity at 35°C and about 75% activity at 40°C Helianthus tuberosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Helianthus tuberosus

pH Range

pH Minimum pH Maximum Comment Organism
5 6 isoform 1-FEH I shows more than 80% activity between pH 5.0 and 6.0 Helianthus tuberosus
5.5 7 isoform 1-FEH II shows more than 50% activity between pH 5.5 and 7.0 Helianthus tuberosus

pI Value

Organism Comment pI Value Maximum pI Value
Helianthus tuberosus isoform 1-FEH II, calculated from amino acid sequence
-
 4.9
Helianthus tuberosus isoform 1-FEH I, calculated from amino acid sequence
-
 6.7

Expression

Organism Comment Expression
Helianthus tuberosus 15% (w/v) PEG treatments slightly inhibit the expression of isoform 1-FEH II in tubers down
Helianthus tuberosus isoform 1-FEH I transcripts accumulate to high levels in the developing leaves and stems of artichoke. The level of the isoform transcript is significantly increased in the stems and tubers of 100 mM NaCl-treated plants up
Helianthus tuberosus the expression levels of isoform 1-FEH II increase in tubers during tuber sprouting. The level of the isoform transcript is significantly increased in the stems and tubers of 100 mM NaCl-treated plants up