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Literature summary for 3.2.1.81 extracted from
- Purification and characterization of an extracellular beta-agarase from Bacillus sp. MK03 (2003), J. Biosci. Bioeng., 95, 328-334.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 2 mM, 76% inhibition | Bacillus sp. (in: Bacteria) |  |
| FeCl2 | 2 mM, 99% inhibition | Bacillus sp. (in: Bacteria) | |
| HgCl2 | 2 mM, 100% inhibition | Bacillus sp. (in: Bacteria) | |
| additional information | not inhibitory: p-chloromercuribenzoate, EDTA | Bacillus sp. (in: Bacteria) | |
| PbCl2 | 2 mM, 98% inhibition | Bacillus sp. (in: Bacteria) | |
| sodium dodecylsulfate | 2 mM, 100% inhibition | Bacillus sp. (in: Bacteria) | |
| ZnCl2 | 2 mM, 96% inhibition | Bacillus sp. (in: Bacteria) | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
1 * 92000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| 113000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
MK03 | - |
| Bacillus sp. (in: Bacteria) MK03 | - |
MK03 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.2 | - |
pH 7.0, 40°C | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| agarose + H2O | - |
Bacillus sp. (in: Bacteria) | neoagarotetraose + ? | main product | ? | |
| agarose + H2O | - |
Bacillus sp. (in: Bacteria) MK03 | neoagarotetraose + ? | main product | ? | |
| neoagarohexaose + H2O | - |
Bacillus sp. (in: Bacteria) | neoagarotetraose + neoagarobiose | - |
? | |
| neoagarohexaose + H2O | - |
Bacillus sp. (in: Bacteria) MK03 | neoagarotetraose + neoagarobiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 92000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
| More | N-terminal amino acid sequence | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable up to | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 8.2 | - |
Bacillus sp. (in: Bacteria) |
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