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Literature summary for 3.2.1.81 extracted from

  • Long, M.; Yu, Z.; Xu, X.
    A novel beta-agarase with high pH stability from marine Agarivorans sp. LQ48 (2010), Mar. Biotechnol., 12, 62-69.
    View publication on PubMed
Search for more literature for 3.2.1.81

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol beta-mercaptoethanol even at a low concentration of 10 mM increases the activity of AgaA by 23% Agarivorans sp. LQ48
dithiothreitol dithiothreitol even at a low concentration of 10 mM increases the activity of AgaA by 21% Agarivorans sp. LQ48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Agarivorans sp. LQ48

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-(2-nitrobenzoic acid) 53% residual activity at 10 mM Agarivorans sp. LQ48
Cu2+ 69% residual activity at 10 mM Agarivorans sp. LQ48
EDTA 69% residual activity at 10 mM Agarivorans sp. LQ48
Mg2+ 65% residual activity at 10 mM Agarivorans sp. LQ48
SDS 71% residual activity at 10 mM Agarivorans sp. LQ48

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ low concentration of Mn2+ have a slight positive effect on the AgaA activity (116% relative activity at 1 mM) Agarivorans sp. LQ48
additional information no significant activation or inhibition of AgaA is observed by Na+, K+, Ca2+, Fe3+, and urea Agarivorans sp. LQ48

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47200
-
 SDS-PAGE Agarivorans sp. LQ48
51200
-
 calculated from amino acid sequence Agarivorans sp. LQ48

Organism

Organism UniProt Comment Textmining
Agarivorans sp. LQ48 C9WIW7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Agarivorans sp. LQ48

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarose + H2O the recombinant enzyme hydrolyzes the beta-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products Agarivorans sp. LQ48 neoagarotetraose + neoagarohexaose
-
 ?
additional information when neoagarotetraose and neoagarohexaose are used as substrates for AgaA, no hydrolysis products are detected Agarivorans sp. LQ48 ?
-
 ?

Synonyms

Synonyms Comment Organism
AgaA
-
 Agarivorans sp. LQ48

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Agarivorans sp. LQ48

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Agarivorans sp. LQ48

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 AgaA still retains more than 95% activity after incubation at pH 3.0-11.0 for 1 h Agarivorans sp. LQ48