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Literature summary for 3.2.2.14 extracted from
- Pyridine nucleotide cycle of Salmonella typhimurium: in vitro demonstration of nicotinamide adenine dinucleotide glycohydrolase, nicotinamide mononucleotide glycohydrolase, and nicotinamide adenine dinucleotide pyrophosphatase activities (1981), J. Bacteriol., 145, 1002-1009.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | addition of 1.4 mM, inhibition 54.7% | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| ADP-ribose | addition of 1-1.4 mM, inhibition 74.4% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| AMP | addition of 1.4 mM AMP, inhibition 92.5% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| D-ribose-5-phosphate | addition of 1.4 mM, inhibition 42.3% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| desamido-NAD+ | addition of 1 mM, inhibition 66.6% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| GMP | addition of 1.4 mM GMP, inhibition 89.4% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| guanosine | addition of 1.4 mM, inhibition 44.6% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NAD+ | greatly inhibited by intracellular levels of NAD+ and reduced NADH, addition of 1 mM NAD+, inhibition 73.2% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NADH | addition of 1 mM, inhibition 68.6% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NADP+ | addition of 1 mM, inhibition 24% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| nicotinamide hypoxanthine dinucleotide | addition of 1 mM nicotinamide hypoxanthine dinucleotide, inhibition 61.4% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | addition of 1.4 mM, inhibition 8.8% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal HCl | addition of 1.4 mM, inhibition 5.2% | Salmonella enterica subsp. enterica serovar Typhimurium | |
| thio-NAD+ | addition of 1 mM, inhibition 63.2% | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide mononucleotide + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.032 | - |
addition of 1.4 mM GTP, inhibition 93.1% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.034 | - |
addition of 1.4 mM AMP, inhibition 92.5% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.049 | - |
addition of 1.4 mM GMP, inhibition 89.4% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.117 | - |
addition of 1.4 mM ADP-ribose, inhibition 74.4% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.122 | - |
addition of 1 mM ADP-ribose, inhibition 74.4% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.128 | - |
addition of 1 mM NAD+, inhibition 73.2% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.15 | - |
addition of 1 mM NADH, inhibition 68.6% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.16 | - |
addition of 1 mM desamido-NAD+, inhibition 66.6% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.176 | - |
addition of 1 mM thio-NAD+, inhibition 63.2% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.186 | - |
addition of 1 mM NHD, inhibition 61.4% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.208 | - |
addition of 1.4 mM adenosine, inhibition 54.7% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.254 | - |
addition of 1.4 mM guanosine, inhibition 44.6% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.264 | - |
addition of 1.4 mM D-ribose-5-phosphate, inhibition 42.3% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.363 | - |
addition of 1 mM NADP+, inhibition 24% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.418 | - |
addition of 1.4 mM pyridoxal 5'-phosphate, inhibition 8.8% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.434 | - |
addition of 1.4 mM pyridoxal HCl, inhibition 5.2% | Salmonella enterica subsp. enterica serovar Typhimurium |
| 0.478 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide mononucleotide + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
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