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Literature summary for 3.2.2.16 extracted from

  • Mishra, V.; Ronning, D.R.
    Crystal structures of the Helicobacter pylori MTAN enzyme reveal specific interactions between S-adenosylhomocysteine and the 5-alkylthio binding subsite (2012), Biochemistry, 51, 9763-9772.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Rosetta cells Helicobacter pylori

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M HEPES pH 7.5 and 25% (w/v) PEG 3350 or 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5 and 30% (v/v) PEG-MME 550 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
D198N inactive Helicobacter pylori
F107A the mutant shows reduced activity compared to the wild type enzyme Helicobacter pylori
H109A the mutant shows reduced activity compared to the wild type enzyme Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 S-adenosyl-L-homocysteine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori
0.014
-
 S-adenosyl-L-homocysteine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
0.019
-
 S-adenosyl-L-homocysteine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
0.033
-
 S-methyl-5'-thioadenosine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
0.037
-
 S-methyl-5'-thioadenosine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
0.039
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-deoxyadenosine + H2O Helicobacter pylori
-
 5-deoxy-D-ribose + adenine
-
 ?
6-amino-6-deoxyfutalosine + H2O Helicobacter pylori
-
 3-{3-[(2R,3S,4R,5R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl}benzoic acid + 9H-purine-6-amine
-
 ?
S-adenosyl-L-homocysteine + H2O Helicobacter pylori
-
 S-ribosyl-L-homocysteine + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O Helicobacter pylori
-
 S-methyl-5-thio-D-ribose + adenine
-
 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori Q9ZMY2
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxyadenosine + H2O
-
 Helicobacter pylori 5-deoxy-D-ribose + adenine
-
 ?
6-amino-6-deoxyfutalosine + H2O
-
 Helicobacter pylori 3-{3-[(2R,3S,4R,5R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl}benzoic acid + 9H-purine-6-amine
-
 ?
S-adenosyl-L-homocysteine + H2O
-
 Helicobacter pylori S-ribosyl-L-homocysteine + adenine
-
 ?
S-methyl-5'-thioadenosine + H2O
-
 Helicobacter pylori S-methyl-5-thio-D-ribose + adenine
-
 ?

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Helicobacter pylori
MTAN
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.44
-
 S-adenosyl-L-homocysteine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
0.74
-
 S-adenosyl-L-homocysteine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
1
-
 S-methyl-5'-thioadenosine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
1.2
-
 S-methyl-5'-thioadenosine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
1.8
-
 S-adenosyl-L-homocysteine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori
3.8
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23
-
 S-adenosyl-L-homocysteine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
27
-
 S-methyl-5'-thioadenosine mutant enzyme F107A, at pH 7.2 and 25°C Helicobacter pylori
37
-
 S-methyl-5'-thioadenosine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
51
-
 S-adenosyl-L-homocysteine mutant enzyme H109A, at pH 7.2 and 25°C Helicobacter pylori
97
-
 S-methyl-5'-thioadenosine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori
170
-
 S-adenosyl-L-homocysteine wild type enzyme, at pH 7.2 and 25°C Helicobacter pylori