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Literature summary for 3.2.2.20 extracted from
- Two DNA glycosylases in Escherichia coli which release primarily 3-methyladenine (1982), Biochemistry, 21, 1162-1169.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Methyladenine | noncompetitive inhibition | Escherichia coli |  |
| apurinic-DNA | - |
Escherichia coli | |
| Double-stranded DNA | - |
Escherichia coli | |
| N-ethylmaleimide | 1 mM, 29% inhibition | Escherichia coli | |
| NaCl | - |
Escherichia coli | |
| spermidine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000014 | - |
3-methyladenine residues in alkylated DNA | pH 7.8, 37°C, release of 3-methyladenine | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulated by 1 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + ? | - |
? | |
| alkylated DNA + H2O | Escherichia coli BW 9062 | - |
3-methyladenine + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli BW 9062 | - |
- |
- |
| no activity in Escherichia coli | - |
mutants tag 1 and tag 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | - |
? | |
| 3-methyladenine residues in alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | - |
? | |
| alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | - |
? | |
| alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + ? | also releases a minmal amount of 3-methylguanine | ? | |
| alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | - |
? | |
| alkylated DNA + H2O | - |
Escherichia coli BW 9062 | 3-methyladenine + ? | also releases a minmal amount of 3-methylguanine | ? | |
| additional information | will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli | ? | - |
? | |
| additional information | will not release 2,6-diamino-4-hydroxy-5-(N-methylformamido)pyrimidine, the alkali-induced derivative of 7-methylguanine, in which the imidazole ring is opened | Escherichia coli BW 9062 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48 | - |
50% inactivation requires 5 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | more active in 0.07 M HEPES-KOH buffer than in 0.07 M Tris-HCl buffer | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
3-Methyladenine | pH 7.8, 37°C | Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | - |
6 |
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