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Literature summary for 3.3.2.6 extracted from

  • Byzia, A.; Haeggstroem, J.Z.; Salvesen, G.S.; Drag, M.
    A remarkable activity of human leukotriene A4 hydrolase (LTA4H) toward unnatural amino acids (2014), Amino Acids, 46, 1313-1320.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.301
-
 L-arginine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contans zinc Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Homo sapiens
-
 leukotriene B4
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-arginine + H2O the best proteinogenic amino acid recognized by the enzyme is L-arginine Homo sapiens ?
-
 ?
L-arginine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-arginine + 7-amino-4-methylcoumarin
-
 ?
L-glutamate-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-glutamate + 7-amino-4-methylcoumarin
-
 ?
L-histidine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-histidine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-lysine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-lysine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-methionine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
L-proline-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-proline + 7-amino-4-methylcoumarin
-
 ?
L-serine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-serine + 7-amino-4-methylcoumarin
-
 ?
L-tyrosine-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-tyrosine + 7-amino-4-methylcoumarin
-
 ?
leukotriene A4 + H2O
-
 Homo sapiens leukotriene B4
-
 ?
additional information no activity with L-Asn, L-Asp, L-Glu, Gly, L-Ile, L-Thr, L-Trp, L-Val, as well as D-amino acids Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
leukotriene A4 hydrolase
-
 Homo sapiens
LTA4H
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.298
-
 L-methionine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% of bovine serum albumin, at 37°C Homo sapiens
0.343
-
 L-leucine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens
0.449
-
 L-proline-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens
0.4706
-
 L-arginine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens
0.617
-
 L-lysine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens
0.747
-
 L-phenylalanine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens
1.497
-
 L-alanine-7-amido-4-methylcoumarin in 250 mM Tris buffer, pH 7.5, containing 500 mM KCl and 0.1% (w/v) of bovine serum albumin, at 37°C Homo sapiens