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Literature summary for 3.4.11.1 extracted from
- A secreted aminopeptidase of Pseudomonas aeruginosa (2001), J. Biol. Chem., 276, 43645-43652.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 0.5 mM, completely inhibited | Pseudomonas aeruginosa |  |
| 1,7-phenanthroline | 0.5 mM, 93% activity | Pseudomonas aeruginosa | |
| 4,7-phenanthroline | 0.5 mM, 95% activity | Pseudomonas aeruginosa | |
| dithiothreitol | 10 mM, completely inhibited | Pseudomonas aeruginosa | |
| EDTA | 10 mM, 60% activity | Pseudomonas aeruginosa | |
| EGTA | 10 mM, 48% activity | Pseudomonas aeruginosa | |
| additional information | not inhibited by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, 3,4-dichloroisocoumarin, tosyl-lysine chloromethylketone, N-methylmaleimide, phosphoramidon | Pseudomonas aeruginosa | |
| N-ethylmaleimide | 5 mM, 95% activity | Pseudomonas aeruginosa | |
| tetraethylene pentamine | 1 mM, completely inhibited | Pseudomonas aeruginosa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | AP 28 | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE, AP 28 | Pseudomonas aeruginosa |
| 56000 | - |
SDS-PAGE, AP 56 | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
strains Habs serotype 1, FRD2 and PAO1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.5-4 units/mg, one unit is the amount of enzyme that causes an increase in optical density at 405 nm of 1 unit/h, substrate 0.6 mM leucine-p-nitroanilide in 1 ml of 0.05 M Tris/HCl, 1 mM CaCl2, pH 8.3 | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucine-p-nitroanilide + H2O | - |
Pseudomonas aeruginosa | L-leucine + p-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AP 28 | - |
Pseudomonas aeruginosa |
| AP 56 | - |
Pseudomonas aeruginosa |
| PaAP | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
conversion to a 28 kDa protein that retained enzyme activity | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
AP 28 | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | - |
Pseudomonas aeruginosa |
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